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[NUSAP1 promotes lung cancer progression by activating AKT/mTOR signaling pathway]

Z Yu1, X M Li2, M Huai3

  • 1Department of Cardiothoracic Medicine, North China Petroleum Administration Bureau General Hospital, Renqiu 062552, China.

Zhonghua Zhong Liu Za Zhi [Chinese Journal of Oncology]
|August 27, 2020
PubMed

Insights

Nucleolar and spindle associated protein 1 (NUSAP1) knockdown inhibits lung cancer cell growth and spread. This occurs by reducing the AKT/mTOR pathway, leading to increased cancer cell death.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Lung cancer remains a leading cause of cancer-related mortality worldwide.
  • Understanding the molecular mechanisms driving lung cancer progression is crucial for developing effective therapies.
  • Nucleolar and spindle associated protein 1 (NUSAP1) has been implicated in cell division and cancer, but its specific role in lung cancer requires further elucidation.

Purpose of the Study:

  • To investigate the inhibitory effects of targeting nucleolar and spindle associated protein 1 (NUSAP1) in lung cancer.
  • To elucidate the underlying molecular mechanisms, particularly the involvement of the AKT/mTOR signaling pathway.

Main Methods:

  • Utilized A549 lung cancer cells transfected with NUSAP1 siRNA.
  • Assessed cell proliferation, migration, invasion, and apoptosis using CCK8, Transwell assays, and flow cytometry.
  • Analyzed protein expression related to apoptosis and the AKT/mTOR pathway via Western blot.

Main Results:

  • NUSAP1 knockdown significantly inhibited proliferation, migration, and invasion of A549 cells.
  • NUSAP1 knockdown significantly increased apoptosis in A549 cells.
  • Downregulation of NUSAP1 led to increased Bax and active-caspase 3, and decreased Bcl-2, P70, and phosphorylation of AKT/mTOR.

Conclusions:

  • NUSAP1 knockdown demonstrates significant anti-cancer effects in lung cancer cells.
  • The observed effects are mediated through the suppression of the AKT/mTOR signaling pathway.
  • Targeting NUSAP1 represents a potential therapeutic strategy for lung cancer treatment.
Keywords:
Cell apoptosisCell proliferationLung neoplasmsNucleolar and spindle associated protein 1

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