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Oleuropein: A Potential Inhibitor for Prostate Cancer Cell Motility by Blocking Voltage-Gated Sodium Channels

Hatice Gumushan Aktas1, Huda Ayan1

  • 1Faculty of Arts & Sciences, Biology Department, Harran University, Sanliurfa, Turkey.

Nutrition and Cancer
|August 27, 2020
PubMed

Insights

Olive leaf extract and oleuropein inhibit prostate cancer cell motility by blocking voltage-gated sodium channels (VGSCs). This suggests oleuropein may be a potential antimetastatic agent for prostate cancer.

Area of Science:

  • Pharmacology
  • Molecular Biology
  • Cancer Research

Background:

  • Metastasis, a hallmark of cancer, involves cell motility.
  • Voltage-gated sodium channels (VGSCs) are implicated in cancer cell migration.
  • Olive leaf extract (OLE) contains bioactive compounds with potential therapeutic properties.

Purpose of the Study:

  • To investigate the anti-motility effects of OLE and oleuropein on prostate cancer cells.
  • To determine if oleuropein blocks VGSCs and inhibits cell motility.
  • To explore the underlying molecular mechanisms, including SCN9A gene expression.

Main Methods:

  • Preparation and analysis of aqueous extract of olive leaves (AOLE) using LC-MS/MS.
  • Assay of AOLE and oleuropein effects on MAT-LyLu prostate cancer cell motility.
  • Determination of non-toxic concentrations of oleuropein.
  • Analysis of SCN9A mRNA expression using quantitative methods.

Main Results:

  • Oleuropein was identified as the primary active compound in AOLE.
  • Oleuropein significantly suppressed the lateral and vertical movement of MAT-LyLu cells at non-toxic concentrations.
  • Oleuropein reduced SCN9A mRNA expression, indicating VGSC pathway involvement.

Conclusions:

  • Oleuropein effectively inhibits prostate cancer cell motility by targeting VGSCs.
  • Oleuropein's mechanism involves the downregulation of SCN9A mRNA expression.
  • Oleuropein shows promise as a potential antimetastatic agent for prostate cancer treatment.

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