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Related Experiment Video

Updated: Dec 10, 2025

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User guide to MiT-TFE isoforms and post-translational modifications.

Hong Nhung Vu1, Ramile Dilshat1, Valerie Fock1

  • 1Department of Biochemistry and Molecular Biology, BioMedical Center, Faculty of Medicine, University of Iceland, Reykjavík, Iceland.

Pigment Cell & Melanoma Research
|August 27, 2020
PubMed
Summary
This summary is machine-generated.

This study provides a manual for cross-referencing amino acids and post-translational modifications in the MiT-TFE transcription factor family. This resource aids research into signaling pathways regulating these key factors in cell fate.

Keywords:
MITFTFEisoformpost-translational modificationstranscription factor

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Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • The microphthalmia-associated transcription factor (MITF) family, including TFEB and TFE3, regulates melanocyte and melanoma cell fate.
  • These factors are crucial for lysosome biogenesis and autophagy, essential cellular recycling processes.
  • Challenges exist in standardizing the indexing of amino acid residues and post-translational modifications across different isoforms and related proteins.

Purpose of the Study:

  • To create a comprehensive resource for cross-referencing amino acids and post-translational modifications within the MiT-TFE transcription factor family.
  • To facilitate standardized research on the signaling pathways affecting MiT-TFE isoforms.
  • To provide protein accession numbers for all isoforms in major genomic databases.

Main Methods:

  • Compilation of data on MITF, TFEB, TFE3, and TFEC isoforms across humans, mice, and zebrafish.
  • Cross-referencing of amino acid residues and known post-translational modifications.
  • Summarization of protein accession numbers from genomic databases.

Main Results:

  • A detailed manual is presented for the MiT-TFE family, covering all isoforms in humans, mice, and zebrafish.
  • The manual includes cross-referenced amino acid positions and post-translational modification sites.
  • Protein accession numbers for each isoform are cataloged.

Conclusions:

  • This resource simplifies the study of signaling pathways impacting MiT-TFE family members.
  • Standardized indexing of post-translational modifications will enhance comparative and functional analyses.
  • Facilitates future research into cell fate determination and cellular recycling mechanisms regulated by MiT-TFE factors.