Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Rational Dual-Site Doping of the Hematite Photoanode Unlocks Efficient Solar Water Splitting.

ACS applied materials & interfaces·2026

Same author

A capacitive-piezoelectric hybrid MEMS microphone with signal fusion for enhancing signal-to-noise ratio.

Microsystems & nanoengineering·2026

Same author

Correction: Yang et al. The Expression Pattern of tRNA-Derived Small RNAs in Adult <i>Drosophila</i> and the Function of <i>tRF-Trp-CCA-014-H3C4</i> Network Analysis. <i>Int. J. Mol. Sci.</i> 2023, <i>24</i>, 6169.

International journal of molecular sciences·2025

Same author

AI‑driven photonic noses: from conventional sensors to cloud‑to-edge intelligent microsystems.

Microsystems & nanoengineering·2025

Same author

Identifying Small-Displacement Stereotypical Behavior of Captive Slow Lorises Based on AnimalYOLO-Bytetrack Network.

Ecology and evolution·2025

Same author

Scutellarin suppresses Mycobacterium tuberculosis-induced pyroptosis in macrophages by inhibiting the HIF-1α-mediated Warburg effect.

Redox report : communications in free radical research·2025

Search research articles

Related Experiment Video

Updated: Dec 10, 2025

Absolute Quantification of Plasma MicroRNA Levels in Cynomolgus Monkeys, Using Quantitative Real-time Reverse Transcription PCR

Absolute Quantification of Plasma MicroRNA Levels in Cynomolgus Monkeys, Using Quantitative Real-time Reverse Transcription PCR

Published on: February 12, 2018

Transcriptome Profiling across Five Tissues of Giant Panda.

Feng Li^1,2, Chengdong Wang³, Zhongxian Xu¹

¹Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China.

Biomed Research International

|August 28, 2020

Summary

This study reveals significant gene expression differences across giant panda organs, identifying novel genes and tissue-specific patterns in the heart, liver, spleen, lung, and kidney for conservation research.

More Related Videos

Quantification of Circulating Pig-Specific DNA in the Blood of a Xenotransplantation Model

Quantification of Circulating Pig-Specific DNA in the Blood of a Xenotransplantation Model

Published on: September 22, 2020

Comprehensive Analysis of Transcription Dynamics from Brain Samples Following Behavioral Experience

Comprehensive Analysis of Transcription Dynamics from Brain Samples Following Behavioral Experience

Published on: August 26, 2014

Related Experiment Videos

Last Updated: Dec 10, 2025

Absolute Quantification of Plasma MicroRNA Levels in Cynomolgus Monkeys, Using Quantitative Real-time Reverse Transcription PCR

Absolute Quantification of Plasma MicroRNA Levels in Cynomolgus Monkeys, Using Quantitative Real-time Reverse Transcription PCR

Published on: February 12, 2018

Quantification of Circulating Pig-Specific DNA in the Blood of a Xenotransplantation Model

Quantification of Circulating Pig-Specific DNA in the Blood of a Xenotransplantation Model

Published on: September 22, 2020

Comprehensive Analysis of Transcription Dynamics from Brain Samples Following Behavioral Experience

Comprehensive Analysis of Transcription Dynamics from Brain Samples Following Behavioral Experience

Published on: August 26, 2014

Area of Science:

Genomics
Comparative transcriptomics
Animal biology

Background:

Gene differential expression studies are crucial for understanding animal life activities.
Previous giant panda research had limited transcriptome data, with only blood and spleen reported.

Purpose of the Study:

To explore transcriptome differences across various giant panda tissues.
To provide comprehensive transcriptome resources for the giant panda's heart, liver, lung, and kidney.

Main Methods:

RNA sequencing of heart, liver, spleen, lung, and kidney from five giant pandas.
Comparative analysis of intertissue gene expression patterns.
Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses, and protein-protein interaction (PPI) network construction.

Main Results:

Generated 194.52 GB of clean base data, identifying 18,701 genes, including 3,492 novel genes.
Identified 921 (heart), 553 (liver), 574 (spleen), 457 (lung), and 638 (kidney) tissue-specific differentially expressed genes (DEGs).
Found similar gene expression patterns between the spleen and lung, and identified key hub genes in each tissue.

Conclusions:

This study presents the first transcriptome data for the giant panda's heart, liver, lung, and kidney.
The findings offer valuable genetic resources for future research on giant pandas, aiding conservation efforts.