Related Experiment Video

Updated: Dec 10, 2025

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer
09:40

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer

Published on: August 2, 2024

3.0K

MicroRNA-195 controls MICU1 expression and tumor growth in ovarian cancer

Geeta Rao1, Shailendra Kumar Dhar Dwivedi2, Yushan Zhang1

  • 1Department of Pathology, The University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA.

EMBO Reports
|August 28, 2020
PubMed

Insights

MicroRNA-195-5p (miR-195) targets MICU1, a protein linked to ovarian cancer progression. Restoring miR-195 levels in ovarian cancer reduces tumor growth and improves survival.

Area of Science:

  • Molecular Biology
  • Oncology
  • Biochemistry

Background:

  • Mitochondrial calcium uptake regulator 1 (MICU1) is elevated in ovarian cancer, promoting glycolysis and chemoresistance.
  • High MICU1 expression correlates with poor clinical outcomes in ovarian cancer patients.
  • Current therapeutic strategies to normalize MICU1 expression are lacking.

Purpose of the Study:

  • To investigate the regulatory role of microRNA-195-5p (miR-195) on MICU1 expression in ovarian cancer.
  • To evaluate the therapeutic potential of restoring miR-195 levels in ovarian cancer models.

Main Methods:

  • Bioinformatic analysis to predict miR-195 targeting of MICU1 mRNA.
  • In vitro studies using ovarian cancer cell lines to assess miR-195 and MICU1 expression.
  • In vivo studies using a human ovarian cancer xenograft model.

Main Results:

  • miR-195 directly targets the 3' UTR of MICU1 mRNA, repressing its expression.
  • miR-195 is significantly under-expressed in ovarian cancer cell lines.
  • Restoring miR-195 normalized MICU1 levels and reversed associated phenotypes.
  • Stable miR-195 expression in vivo reduced tumor growth, increased doubling time, and improved survival.

Conclusions:

  • miR-195 acts as a tumor suppressor by regulating MICU1 in ovarian cancer.
  • Restoring miR-195 expression offers a potential therapeutic strategy to normalize MICU1, reversing glycolysis and chemoresistance.
  • Targeting miR-195 could improve patient outcomes in ovarian cancer.
Keywords:
MICU1/CBARA1Ovarian cancerchemoresistanceglycolysismiR-195

More Related Videos

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

7.9K
Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library08:40

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

17.9K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.5K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
23.6K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.5K