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Related Experiment Videos

Fermentation with immobilized cell cultures.

R G Werner1, W Merk, F Walz

  • 1Dr. Karl Thomae GmbH, Bioengineering Techniques, Biberach an der Riss, Fed. Rep. of Germany.

Arzneimittel-Forschung
|February 1, 1988
PubMed
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Immobilized cell culture systems, including solid bed bioreactors, enable efficient production of therapeutic proteins like monoclonal antibodies. These systems allow continuous media exchange and are scalable for industrial applications.

Area of Science:

  • Biotechnology
  • Bioprocess Engineering
  • Cell Culture Technology

Background:

  • Various immobilized cell culture systems exist for producing monoclonal antibodies and recombinant proteins.
  • These systems offer advantages such as reduced media volumes and continuous processing capabilities.
  • Scalability is a key consideration, with some systems like hollow fiber and Opticell facing limitations.

Purpose of the Study:

  • To evaluate and compare different immobilized cell culture systems for biopharmaceutical production.
  • To highlight the advantages of scalable bioreactor designs for industrial applications.
  • To demonstrate the successful application of a specific system for producing key therapeutic proteins.

Main Methods:

  • Development and application of immobilized cell culture systems, including microcarrier, fluidized bed, and solid bed bioreactors.

Related Experiment Videos

  • Continuous media exchange for optimized product synthesis and harvest.
  • Utilizing wire springs as a support matrix in solid bed bioreactors for cell immobilization.

    • Main Results:

    • Solid bed bioreactors are suitable for large-scale production, unlike some other systems.
    • The solid bed bioreactor requires minimal cell anchoring manipulation and allows for in situ cleaning and bead reuse.
    • Successful production processes were established for interferon beta, monoclonal antibodies for interferon alfa, and recombinant human tissue plasminogen activator.

    Conclusions:

    • Solid bed bioreactors represent a scalable and efficient immobilized cell culture system for biopharmaceutical manufacturing.
    • The system's design facilitates ease of use, reusability, and in situ cleaning, reducing operational complexity.
    • This technology enables the robust production of critical therapeutic proteins, supporting advancements in medicine.