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4D-bioprinted silk hydrogels for tissue engineering.

Soon Hee Kim1, Ye Been Seo1, Yeung Kyu Yeon1

  • 1Nano-Bio Regenerative Medical Institute, College of Medicine, Hallym University, Chuncheon, 24252, Republic of Korea.

Biomaterials
|August 29, 2020
PubMed
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This summary is machine-generated.

This study introduces a biocompatible four-dimensional (4D) bioprinting system using silk fibroin hydrogels. The novel system successfully created trachea tissue in rabbits, demonstrating potential for tissue engineering and clinical applications.

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Four-dimensional (4D) printing is a promising biofabrication technology but faces challenges in biocompatibility and multi-component printability.
  • Existing 4D bioprinting applications are largely theoretical or limited to in vitro studies, lacking established implantable targets.
  • There is a need for advanced 4D bioprinting systems that are cell-friendly, biocompatible, and capable of creating complex, functional tissues.

Purpose of the Study:

  • To develop a novel, cell-friendly, and biocompatible 4D bioprinting system.
  • To demonstrate the capability of creating multi-cellular constructs with controlled shape changes.
  • To evaluate the in vivo efficacy of 4D bioprinted tissue for tracheal regeneration.

Main Methods:

Keywords:
4D bioprintingDigital light processingHeterogenous tissueOsmotic pressureSilk fibroinTrachea

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  • Utilized digital light processing (DLP) and a photocurable silk fibroin (Sil-MA) hydrogel for 4D bioprinting.
  • Incorporated more than two cell types into the hydrogel constructs.
  • Employed finite element analysis (FEA) simulations to predict and analyze shape changes.
  • Implanted 3D printed bilayered Sil-MA hydrogel trachea mimetic tissue into rabbit tracheas.

Main Results:

  • The 4D bioprinting system demonstrated biocompatibility and multi-component printability.
  • Controlled shape changes of the hydrogel constructs were achieved by modulating material properties.
  • Implanted trachea mimetic tissue integrated naturally with the host, forming both epithelium and cartilage.
  • FEA simulations aided in understanding the complex structural changes.

Conclusions:

  • The developed 4D bioprinting system offers a cell-friendly and biocompatible approach for creating tissue-mimetic scaffolds.
  • This technology shows significant potential for advancing tissue engineering and clinical applications, particularly in regenerative medicine.
  • The successful in vivo implantation and regeneration of tracheal tissue highlight the system's clinical relevance.