Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Potentiometry: Membrane Electrodes01:15

Potentiometry: Membrane Electrodes

1.4K
Membrane electrodes, also known as p-ion electrodes, use membranes that selectively interact with free analyte ions, generating a potential difference across the membrane. The resulting membrane potential, known as the asymmetry potential, is not zero even when analyte concentrations on both sides of the membrane are equal. The membrane's response is typically not selective to a single analyte but proportional to the concentration of all ions in the sample solution capable of interacting at...
1.4K
Amperometry: Overview01:10

Amperometry: Overview

1.4K
Amperometry is a technique commonly used to measure the concentration of specific analytes in a solution by monitoring the electric current generated during an electrochemical reaction. It involves applying a constant potential between a working electrode and a reference electrode to measure the resulting current, which is proportional to the concentration of the analyte. The Clark oxygen electrode operates based on this principle of amperometry. It consists of a cathode and an anode enclosed...
1.4K
Electrodes: Overview01:17

Electrodes: Overview

2.4K
 Electrochemical measurements are conducted in an electrochemical cell composed of various components that control and measure the current and potential. One fundamental component is electrodes, conductive materials that enable electron transfer reactions at their surfaces.
There are two main types of electrodes in electrochemical cells. The first type, known as the working or indicator electrode, has a potential that is sensitive to the analyte's concentration and reacts to changes in...
2.4K
Interfacial Electrochemical Methods: Overview01:06

Interfacial Electrochemical Methods: Overview

683
Interfacial electrochemical methods focus on the phenomena occurring at the boundary between an electrode and a solution, as opposed to bulk methods that concentrate on the solution's overall properties. These interfacial methods are classified as either static or dynamic based on the presence of a nonzero current in the electrochemical cell and the consistency of analyte concentrations. Static methods, such as potentiometry, measure the cell's potential without any significant current...
683

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Direct electron transfer-type NADH regeneration flow reactor with recombinant diaphorase subunit of formate dehydrogenase 1.

Bioelectrochemistry (Amsterdam, Netherlands)·2026
Same author

Structure-guided engineering of membrane-binding regions for surfactant-free solubilization of direct electron transfer-type alcohol dehydrogenase.

Chemical communications (Cambridge, England)·2026
Same author

Ion transport across bilayer lipid membranes between two aqueous phases in the presence of iodide and triiodide ions.

Physical chemistry chemical physics : PCCP·2025
Same author

Elephant seals in poorer body condition trade sleep for foraging.

Current biology : CB·2025
Same author

Roles of Labile Copper Coordinated by Histidine in Reductive Inactivation of Copper Efflux Oxidase.

Inorganic chemistry·2025
Same author

Correction to "Self-Tuning Reaction Conditioning of a Paper-Based Electrochemical Biosensor for Acetaldehyde Detection".

ACS omega·2025

Related Experiment Video

Updated: Dec 10, 2025

Waste Water Derived Electroactive Microbial Biofilms: Growth, Maintenance, and Basic Characterization
11:58

Waste Water Derived Electroactive Microbial Biofilms: Growth, Maintenance, and Basic Characterization

Published on: December 29, 2013

13.9K

Development Perspective of Bioelectrocatalysis-Based Biosensors.

Taiki Adachi1, Yuki Kitazumi1, Osamu Shirai1

  • 1Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo, Kyoto 606-8502, Japan.

Sensors (Basel, Switzerland)
|August 30, 2020
PubMed
Summary
This summary is machine-generated.

This review explores bioelectrocatalysis for electrochemical biosensors, detailing enzyme-electrode interactions and summarizing amperometric biosensor applications. It also introduces novel approaches for enhanced enzymatic activity and absolute analyte determination.

Keywords:
current–potential curvemass-transfer-controlled amperometric responsemulti-enzymatic cascadesmultianalyte detectionpotentiometric coulometry

More Related Videos

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation
13:42

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

12.3K
Characterizing Mediated Extracellular Electron Transfer in Lactic Acid Bacteria with a Three-Electrode, Two-Chamber Bioelectrochemical System
10:23

Characterizing Mediated Extracellular Electron Transfer in Lactic Acid Bacteria with a Three-Electrode, Two-Chamber Bioelectrochemical System

Published on: August 23, 2024

1.5K

Related Experiment Videos

Last Updated: Dec 10, 2025

Waste Water Derived Electroactive Microbial Biofilms: Growth, Maintenance, and Basic Characterization
11:58

Waste Water Derived Electroactive Microbial Biofilms: Growth, Maintenance, and Basic Characterization

Published on: December 29, 2013

13.9K
Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation
13:42

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

12.3K
Characterizing Mediated Extracellular Electron Transfer in Lactic Acid Bacteria with a Three-Electrode, Two-Chamber Bioelectrochemical System
10:23

Characterizing Mediated Extracellular Electron Transfer in Lactic Acid Bacteria with a Three-Electrode, Two-Chamber Bioelectrochemical System

Published on: August 23, 2024

1.5K

Area of Science:

  • Electrochemistry
  • Biotechnology
  • Biosensor Technology

Background:

  • Bioelectrocatalysis leverages redox enzymes for electrode reactions, forming a core concept in electrochemical biosensors.
  • Understanding mediated and direct electron transfer is crucial for bioelectrocatalytic reaction mechanisms.

Purpose of the Study:

  • To theoretically describe bioelectrocatalytic reactions.
  • To review amperometric biosensors utilizing multi-enzymatic cascades for multianalyte detection.
  • To introduce novel biosensor concepts for enhanced performance and absolute determination.

Main Methods:

  • Theoretical analysis of mediated and direct electron transfer in bioelectrocatalysis.
  • Review of amperometric biosensor designs for enzyme cascades and multianalyte sensing.
  • Exploration of mass-transfer-controlled amperometry and potentiometric coulometry.

Main Results:

  • Established fundamental characteristics of bioelectrocatalytic reactions.
  • Summarized applications of amperometric biosensors in complex detection scenarios.
  • Proposed new biosensor strategies for calibration-free and absolute measurements.

Conclusions:

  • Bioelectrocatalysis is fundamental to advancing electrochemical biosensor capabilities.
  • Novel approaches offer potential for simplified, more accurate biosensing.
  • Further research in these areas promises significant improvements in biosensor technology.