Related Experiment Video
Updated: Dec 10, 2025

Lensless Fluorescent Microscopy on a Chip
Published on: August 17, 2011
Lensless light-field imaging through diffuser encoding
Zewei Cai1,2, Jiawei Chen2, Giancarlo Pedrini1
1Institut für Technische Optik, Universität Stuttgart, Pfaffenwaldring 9, 70569 Stuttgart, Germany.
Abstract:
Microlens array-based light-field imaging has been one of the most commonly used and effective technologies to record high-dimensional optical signals for developing various potential high-performance applications in many fields. However, the use of a microlens array generally suffers from an intrinsic trade-off between the spatial and angular resolutions. In this paper, we concentrate on exploiting a diffuser to explore a novel modality for light-field imaging. We demonstrate that the diffuser can efficiently angularly couple incident light rays into a detected image without needing any lens. To characterize and analyse this phenomenon, we establish a diffuser-encoding light-field transmission model, in which four-dimensional light fields are mapped into two-dimensional images via a transmission matrix describing the light propagation through the diffuser. Correspondingly, a calibration strategy is designed to flexibly determine the transmission matrix, so that light rays can be computationally decoupled from a detected image with adjustable spatio-angular resolutions, which are unshackled from the resolution limitation of the sensor. The proof-of-concept approach indicates the possibility of using scattering media for lensless four-dimensional light-field recording and processing, not just for two- or three-dimensional imaging.
Related Concept Videos
Confocal Fluorescence Microscopy
Super-resolution Fluorescence Microscopy
Phase Contrast and Differential Interference Contrast Microscopy
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Light Acquisition
Imaging Biological Samples with Optical Microscopy
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Focusing of Light in the Eye

