Aptamer-Mediated Reversible Transactivation of Gene Expression by Light
Christian Renzl1, Ankana Kakoti1, Günter Mayer1,2
1LIMES, University of Bonn, Gerhard-Domagk-Str. 1, 53121, Bonn, Germany.
Abstract:
The investigation and manipulation of cellular processes with subcellular resolution requires non-invasive tools with spatiotemporal precision and reversibility. Building on the interaction of the photoreceptor PAL with an RNA aptamer, we describe a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression. This platform significantly reduces the coding space required for genetic manipulation and provides a strong on-switch with almost no residual activity in the dark. It adds to the current set of modular building blocks for synthetic biological circuit design and is broadly applicable.
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