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Optogenetic Stimulation of the Central Amygdala Using Channelrhodopsin
Anna S Knes1,2, Charlotte M Freeland1,3, Mike J F Robinson1,2
1Neuroscience & Behavior Program, Wesleyan University, Middletown, CT, USA.
Methods in Molecular Biology (Clifton, N.J.)
|September 1, 2020
Abstract:
Optogenetics allows for the targeted temporary inhibition or stimulation of specific brain regions in vivo with precise temporal resolution. Here, we describe the steps to perform intracranial optogenetic surgery in rodents as well as instructions to build an optogenetic headcap and set up an optogenetic testing environment to conduct experiments. Behavioral studies have implemented these methods to stimulate the central amygdala (CeA) to create an addictive-like preference for reward.

