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Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

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Highly Multiplex Targeted Proteomics Enabled by Real-Time Chromatographic Alignment.

Philip M Remes1, Ping Yip1, Michael J MacCoss2

  • 1Thermo Fisher Scientific, 355 River Oaks Parkway, San Jose, California 95134, United States.

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|September 2, 2020
PubMed
Summary
This summary is machine-generated.

This study introduces real-time chromatographic alignment to improve targeted mass spectrometry. This method enhances throughput and sensitivity for large-scale proteomics studies.

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Area of Science:

  • Proteomics
  • Analytical Chemistry
  • Biotechnology

Background:

  • Targeted mass spectrometry offers high-quality quantitative data but suffers from low throughput.
  • Challenges include logistical issues and analyte retention time drift in liquid chromatography (LC) columns.
  • This limits the number of targets and throughput in proteomics assays.

Purpose of the Study:

  • To address the limitations of targeted mass spectrometry by developing a method for real-time chromatographic alignment.
  • To enable higher throughput and increased target multiplexing in proteomics experiments.
  • To facilitate large cohort studies without compromising sensitivity and specificity.

Main Methods:

  • A novel method for real-time chromatographic alignment was developed.
  • The method utilizes periodic untargeted survey scans for alignment during subsequent experiments.
  • Applied to a 56-minute LC-gradient HeLa digest assay targeting 1489 peptides.

Main Results:

  • The real-time alignment method enables narrower scheduled retention time windows.
  • This allows for increased target inclusion or longer allocation time per target, enhancing sensitivity.
  • Demonstrated application to a 1489-target assay.

Conclusions:

  • Real-time chromatographic alignment significantly improves throughput and sensitivity in targeted mass spectrometry.
  • The method facilitates the analysis of thousands of peptides within an hour-long experiment.
  • Potential for further improvements and increased target multiplexing in future applications.