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A drug screening toolkit based on the -1 ribosomal frameshifting of SARS-CoV-2
Yanqiong Chen1,2,3, Huan Tao4, Silan Shen5,3
1Laboratory of Human Disease and Immunotherapies, West China Hospital, Sichuan University, Chengdu, China.
Abstract:
The -1 ribosomal frameshifting is vital for the translation of the open reading frame (ORF)1b in SARS-CoV-2. The products of ORF1b participate in viral replication. Therefore, changing the frameshift frequency reduces the survival of the virus. This study aimed to successfully develop a toolkit for screening antiviral drugs. Finally, the FDA-approved drug library was screened, revealing that ivacaftor and (-)-Huperzine A worked well in changing the -1 ribosomal frameshifting of SARS-CoV-2 in vitro.
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