Related Experiment Video
Updated: Dec 10, 2025
A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
Published on: April 11, 2014
Expression of Ascorbate Peroxidase Derived from Cyanidioschyzon merolae in Mammalian Cells
Suzuro Hitomi1, Shoichiro Kokabu2, Ken-Ichiro Matsumoto3
1Division of Physiology, Kyushu Dental University, Fukuoka, Japan hitomi.suzuro@nihon-u.ac.jp.
Background/Aim:
Ascorbate peroxidase (APX) derived from Cyanidioschyzon merolae, a primitive red alga living in high temperature and acidic environments, has greater anti-oxidative capacity than similar peroxidases occurring in other plants. In the present study, we examined whether expression of Cyanidioschyzon merolae-derived APX (cAPX) in mammalian cells increases cellular anti-oxidative capacity.
Materials And Methods:
The cAPX gene was introduced into the mouse fibroblast-like cell line C3H10T1/2. Production of reactive oxygen species (ROS) and/or cell viability was assessed after heat, H2O2 and acid stimulation.
Results:
Heat and H2O2 stimulation resulted in ROS production. cAPX-expressing cells were more tolerant to oxidative stress induced by heat, H2O2 and acid stimulations than control cells lacking cAPX.
Conclusion:
Introduction of cAPX increases the anti-oxidative capacity in mammalian cells.
