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Published on: May 30, 2017
Molecular diversity in pathogenic variants of vibrio parahaemolyticus in Peru
Junior Caro-Castro1,2, Orson Mestanza1, Willi Quino1
1Instituto Nacional de Salud, Lima, Perú.
Abstract:
During the period from 1995 to 2017, in order to determine the diversity of Vibrio parahaemolyticus pathogenic variants in Peru, 102 Peruvian genomes (97 from a hospital setting and 5 from an out-of-hospital setting) were analyzed using the multilocus typification scheme and BLASTn in the search for virulence genes. Fifteen different sequence types were identified. It was found that the ST3 genotype, which is found in the pandemic clone, was the most abundant, with 52% (n=53); followed by ST120, with 23.5% (n=24); and the CC345 clonal complex, with 11.8% (n=12). A total of 89 analyzed strains presented genes encoding the pathogenicity island VpaI-7 (87.3%), while 96 presented the tdh gene (94.1%), and 6 the trh gene (5.9%). The ST3 genotype was the predominant one during the evaluated period, this genotype was the cause of a major outbreak in Peru's past history. Other pathogenic genotypes found represent a latent public health risk associated with seafood consumption.
Insights
Vibrio parahaemolyticus pathogenic variants in Peru were analyzed between 1995-2017. The ST3 genotype, linked to past outbreaks, was most common, posing a public health risk from seafood.
Area of Science:
- Microbiology
- Genomics
- Public Health
Background:
- Vibrio parahaemolyticus is a significant cause of seafood-borne gastroenteritis globally.
- Understanding the genetic diversity and virulence factors of V. parahaemolyticus is crucial for public health surveillance.
Purpose of the Study:
- To characterize the genetic diversity and identify virulence genes of Vibrio parahaemolyticus strains in Peru from 1995 to 2017.
- To assess the public health implications of identified V. parahaemolyticus genotypes.
Main Methods:
- Analysis of 102 Peruvian V. parahaemolyticus genomes using multilocus sequence typing.
- Detection of virulence genes, including the pathogenicity island VpaI-7, tdh, and trh, using BLASTn.
Main Results:
- Fifteen distinct sequence types (STs) were identified among the analyzed strains.
- ST3, associated with a pandemic clone and past outbreaks, was the most prevalent (52%).
- High prevalence of the VpaI-7 (87.3%) and tdh (94.1%) genes was observed, while the trh gene was less common (5.9%).
Conclusions:
- The ST3 genotype was the predominant V. parahaemolyticus variant in Peru during the study period.
- The presence of other pathogenic genotypes highlights a persistent public health risk associated with seafood consumption in the region.
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