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Activity-Based Protein Profiling (ABPP) of Oxidoreductases
Rita Fuerst1, Rolf Breinbauer1,2
1Institute of Organic Chemistry, Graz University of Technology, Stremayrgasse 9, 8010, Graz, Austria.
Chembiochem : a European Journal of Chemical Biology
|September 4, 2020
Summary
Activity-based protein profiling (ABPP) is a key proteomic tool. This review highlights ABPP for oxidoreductases, noting a gap in probes for reductases, presenting future research opportunities.
Area of Science:
- Biochemistry
- Proteomics
- Chemical Biology
Background:
- Activity-based protein profiling (ABPP) is a powerful technique for studying protein function in cells.
- ABPP has been widely applied to enzyme classes like hydrolases but less so to oxidoreductases.
- Oxidoreductases play crucial roles in cellular redox homeostasis and metabolism.
Purpose of the Study:
- To review the current state of ABPP for oxidoreductases.
- To discuss the scope and limitations of existing ABPP methods for this enzyme class.
- To identify future research directions in the ABPP of oxidoreductases.
Main Methods:
- Literature review of published ABPP studies on oxidoreductases.
- Analysis of probe development and application for different oxidoreductase subclasses.
- Discussion of challenges and opportunities in the field.
Main Results:
- Several ABPP probes have been developed for oxidases, enabling functional studies.
- Activity-based protein profiling of reductases remains largely unexplored.
- Existing methods for oxidoreductase ABPP have specific limitations.
Conclusions:
- ABPP is a valuable tool for studying oxidoreductases, but significant gaps exist.
- The lack of ABPP probes for reductases represents a major unmet need and a promising area for future research.
- Further development of ABPP strategies for oxidoreductases will enhance our understanding of redox biology.
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