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lncRNA LSINCT5 Regulates miR-20a-5p/XIAP to Inhibit the Growth and Metastasis of Osteosarcoma Cells

Shi-An Liao1, Jian Guan2, Hao Mo2

  • 1Department of Spine and Osteopathy Ward, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Province, People's Republic of China.

Oncotargets and Therapy
|September 5, 2020
PubMed
Abstract

Insights

Long non-coding RNA LSINCT5 promotes osteosarcoma growth and metastasis by acting as a sponge for microRNA-20a-5p, ultimately upregulating XIAP. This finding highlights LSINCT5 as a potential oncogene and therapeutic target in osteosarcoma.

Area of Science:

  • Molecular Biology
  • Oncology
  • RNA Biology

Background:

  • Non-coding RNAs (ncRNAs), including long non-coding RNAs (lncRNAs) and microRNAs (miRNAs), are critical regulators in osteosarcoma (OS).
  • Previous research identified microRNA-20a-5p (miR-20a-5p) negatively regulating XIAP, an oncogene in OS.
  • The upstream lncRNA regulators of the miR-20a-5p/XIAP axis in OS remained unclear.

Purpose of the Study:

  • To investigate the role of lncRNA LSINCT5 in osteosarcoma.
  • To elucidate the regulatory mechanism of LSINCT5 in the context of miR-20a-5p and XIAP.
  • To determine the potential of LSINCT5 as a therapeutic target in osteosarcoma.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) and Western blot (WB) to assess gene and protein expression.
  • Cell proliferation, migration, and invasion assays (CCK-8, Transwell) to evaluate cellular functions.
  • Dual luciferase reporter assays, RNA immunoprecipitation (RIP), and RNA pull-down experiments to confirm molecular interactions.
  • In vivo tumor allograft models to assess the effect on tumor growth.

Main Results:

  • LSINCT5 expression was elevated in OS tissues and correlated with poor prognosis.
  • Inhibition of LSINCT5 suppressed OS cell proliferation and metastasis.
  • LSINCT5 functioned as a miR-20a-5p sponge, regulating XIAP expression and thereby influencing OS cell behavior.
  • LSINCT5 promoted OS tumor growth in vivo.

Conclusions:

  • LncRNA LSINCT5 acts as an oncogene in osteosarcoma.
  • LSINCT5 promotes OS growth and metastasis by upregulating XIAP through a competitive endogenous RNA (ceRNA) mechanism involving miR-20a-5p.
  • LSINCT5 represents a potential therapeutic target for osteosarcoma treatment.
Keywords:
LSINCT5growthinvasionlncRNAmiR-20a-5posteosarcoma

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