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Nonlinear multispectral imaging for tumor delineation.

Elena Beletkaia1, Behdad Dashtbozorg1, Rubin G Jansen2

  • 1Netherlands Cancer Institute, Department of Surgery, Amsterdam, Netherlands.

Journal of Biomedical Optics
|September 5, 2020
PubMed
Summary
This summary is machine-generated.

Rapid microscopic analysis using nonlinear imaging techniques accurately identifies tumor boundaries in excised breast tissue. This method offers real-time feedback, potentially improving surgical outcomes by enabling immediate re-excision if needed.

Keywords:
Ramanclassificationfluorescencehyperspectraltissue characterization

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Area of Science:

  • Biomedical Optics
  • Surgical Pathology
  • Medical Imaging

Background:

  • Accurate assessment of tumor margins during breast-preserving surgery is critical.
  • Current methods for analyzing excised tissue are too slow, delaying feedback and potentially requiring repeat surgeries.
  • Immediate feedback on tumor residue could significantly improve surgical success rates.

Purpose of the Study:

  • To develop a fast microscopic analysis method for excised tissue.
  • To enable real-time feedback during breast-preserving tumor surgery.
  • To accurately delineate tumor boundaries directly on excised tissue in or near the operating theatre.

Main Methods:

  • Demonstration of combined nonlinear imaging techniques: hyperspectral coherent anti-Stokes Raman scattering (CARS), second harmonic generation (SHG), and two-photon excited fluorescence (TPF).
  • Application of these techniques at selected wavelengths on excised patient breast tissue.
  • Microscopy in the epi-direction on thick tissue slices.

Main Results:

  • The study achieved a sensitivity of 0.87 and a specificity of 0.95 in delineating tumor boundaries.
  • Each nonlinear imaging signal demonstrated discriminatory power.
  • The accuracy of the information was verified to be independent of sample treatment.

Conclusions:

  • Nonlinear multispectral imaging accurately determines tumor boundaries in excised breast tissue.
  • This microscopy technique, applied directly to thick tissue slices, moves closer to clinical implementation.
  • Real-time margin assessment could enhance the efficacy of breast-preserving tumor surgeries.