Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sequence and expression of mouse gamma-renin.

C C Drinkwater1, B A Evans, R I Richards

  • 1Howard Florey Institute of Experimental Physiology and Medicine, University of Melbourne, Parkville, Victoria, Australia.

The Journal of Biological Chemistry
|June 25, 1988
PubMed
Summary

Mouse gamma-renin, a glandular kallikrein, exhibits an unusual Leu-Leu cleavage pattern. Researchers identified its gene and cDNA, analyzing its amino acid sequence and tissue expression to understand this unique enzymatic activity.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Corrigendum to "Did the UK's public health shielding policy protect the clinically extremely vulnerable during the COVID-19 pandemic in wales? Results of EVITE immunity, a linked data retrospective study" [Public Health 218 (2023) 12-20].

Public health·2023
Same author

Did the UK's public health shielding policy protect the clinically extremely vulnerable during the COVID-19 pandemic in Wales? Results of EVITE Immunity, a linked data retrospective study.

Public health·2023
Same author

Effects of an air pollution personal alert system on health service usage in a high-risk general population: a quasi-experimental study using linked data.

Journal of epidemiology and community health·2016
Same author

Patent foramen ovale causing stroke in an elderly woman.

Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association·2015
Same author

The Concise Guide to PHARMACOLOGY 2013/14: overview.

British journal of pharmacology·2014
Same author

Modeling and analysis of repeat RNA toxicity in Drosophila.

Methods in molecular biology (Clifton, N.J.)·2013

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Enzymology

Background:

  • Glandular kallikreins are serine proteases that activate growth factors and hormones.
  • Most kallikreins cleave peptide bonds at basic residues.
  • Mouse gamma-renin displays a distinct cleavage pattern, similar to renin, at a Leu-Leu bond.

Purpose of the Study:

  • To identify and sequence the gene and cDNA encoding mouse gamma-renin.
  • To determine the complete predicted amino acid sequence of gamma-renin.
  • To investigate the potential residues responsible for its unusual cleavage specificity.
  • To identify the mouse tissues expressing gamma-renin.

Main Methods:

  • Gene and cDNA sequencing.
  • Amino acid sequence prediction and analysis.

Related Experiment Videos

  • Design and use of a gene-specific oligodeoxyribonucleotide probe.
  • Tissue expression analysis via hybridization.

    • Main Results:

    • The gene and cDNA for mouse gamma-renin were identified and sequenced.
    • The complete amino acid sequence was predicted, highlighting residues potentially involved in its unique cleavage.
    • A gene-specific probe was developed, enabling the determination of gamma-renin expression in various mouse tissues.

    Conclusions:

    • Mouse gamma-renin possesses a unique enzymatic activity within the kallikrein family due to its Leu-Leu bond cleavage.
    • Structural analysis provides insights into the mechanism of this unusual proteolytic specificity.
    • Understanding gamma-renin's tissue distribution is crucial for elucidating its physiological roles.