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Updated: Dec 10, 2025

A Multi-Omics Extraction Method for the In-Depth Analysis of Synchronized Cultures of the Green Alga Chlamydomonas reinhardtii
Published on: August 8, 2019
Green choline amino acid ionic liquids aqueous two-phase extraction coupled with synchronous fluorescence
Qi Li1, Wei Liu1, Xiashi Zhu2
1College of Chemistry & Chemical Engineering, Yangzhou University, Yangzhou University, Yangzhou, 225002, PR China.
Abstract:
A novel aqueous two-phase extraction method has been established for the determination of polycyclic aromatic hydrocarbons in water sample. This method was based on the extraction of naphthalene and pyrene from water by means of choline amino acid ionic liquids aqueous two-phase system and their determination by synchronous fluorescence spectroscopy. In synchronous fluorescence spectroscopy, the fluorescence peaks of naphthalene and pyrene were completely separated to meet the requirement of simultaneous determination. For this method, good linear calibration curves of naphthalene and pyrene were obtained in the range of 0.50-10.0, 0.05-5.0 μg mL-1, respectively, and limits of detection were 0.211, 0.012 μg mL-1, respectively. The proposed method was successfully applied for the simultaneous determination of naphthalene and pyrene in water samples, which was considered as an excellent green analysis according Analytical Eco-Scale.
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