Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Osteoclasts in Bone Remodeling

Osteoclasts in Bone Remodeling

Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Bubble-raft inspired shape-assembly in flying robot swarm for uniform formation and obstacle traversal.

Communications engineering·2026

Same author

Development and immunoprotective evaluation of a thermostable mRNA vaccine targeting Vibrio harveyi flagellin in the large yellow croaker (Larimichthys crocea).

Fish & shellfish immunology·2026

Same author

The MAPK Pathway Coordinates an Immunosuppressive Microenvironment in Colorectal Cancer: A Single-Cell Guided Prognostic Model.

Cancer informatics·2026

Same author

Decorin promotes nascent proteoglycan retention in cartilage matrix by strengthening collagen II-aggrecan integration.

Acta biomaterialia·2026

Same author

FKBP9 Enhances Colon Cancer Cell Proliferation by Inhibiting GPX4-Mediated Ferroptosis.

Cancer medicine·2026

Same author

Targeting immunoglobulin superfamily member 9 (IGSF9) to overcome acute myeloid leukemia resistance to CAR-T therapy.

Journal of experimental & clinical cancer research : CR·2026

Search research articles

Related Experiment Video

Updated: Dec 9, 2025

Propagation of Dental and Respiratory Cells and Organs in Microgravity

Propagation of Dental and Respiratory Cells and Organs in Microgravity

Published on: May 25, 2021

Comprehensive circRNA expression profile and function network in osteoblast-like cells under simulated microgravity.

Zhen Cao¹, Yang Zhang², Shuping Wei²

¹College of Biotechnology of Guilin Medical University, Guilin 541199, China.

|September 5, 2020

Summary

This summary is machine-generated.

Circular RNAs (circRNAs) are crucial in osteoblast differentiation under microgravity. This study identified key circRNAs and their regulatory roles, offering new insights into bone formation mechanisms in space.

Keywords:

Osteoblast differentiation RNA-seq Simulated microgravity circRNA

More Related Videos

Culturing Lymphocytes in Simulated Microgravity Using a Rotary Cell Culture System

Culturing Lymphocytes in Simulated Microgravity Using a Rotary Cell Culture System

Published on: August 25, 2022

Using Real-Time Cell Metabolic Flux Analyzer to Monitor Osteoblast Bioenergetics

Using Real-Time Cell Metabolic Flux Analyzer to Monitor Osteoblast Bioenergetics

Published on: March 1, 2022

Related Experiment Videos

Last Updated: Dec 9, 2025

Propagation of Dental and Respiratory Cells and Organs in Microgravity

Propagation of Dental and Respiratory Cells and Organs in Microgravity

Published on: May 25, 2021

Culturing Lymphocytes in Simulated Microgravity Using a Rotary Cell Culture System

Culturing Lymphocytes in Simulated Microgravity Using a Rotary Cell Culture System

Published on: August 25, 2022

Using Real-Time Cell Metabolic Flux Analyzer to Monitor Osteoblast Bioenergetics

Using Real-Time Cell Metabolic Flux Analyzer to Monitor Osteoblast Bioenergetics

Published on: March 1, 2022

Area of Science:

Molecular Biology
Cell Biology
Biotechnology

Background:

Circular RNAs (circRNAs) are novel non-coding RNAs with critical roles in disease pathogenesis.
Their function in osteoblast differentiation under microgravity is largely unexplored.
This study investigates circRNAs in osteoblasts responding to simulated microgravity.

Purpose of the Study:

To investigate the roles and mechanobiological response of circRNAs in osteoblasts under simulated microgravity.
To identify key circRNAs and their regulatory networks involved in osteogenic differentiation.
To provide a molecular understanding of circRNA involvement in bone formation during microgravity exposure.

Main Methods:

RNA transcriptome sequencing (RNA-seq) to profile circRNA and mRNA expression in MC3T3-E1 cells under simulated microgravity.
Quantitative real-time polymerase chain reaction (qRT-PCR) for validation of selected RNA transcripts.
Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses for functional enrichment.

Main Results:

Significant differential expression of 427 circRNAs and 1912 mRNAs in simulated microgravity compared to control.
Parental genes of differentially expressed RNAs enriched in anatomical structure morphogenesis and protein binding.
KEGG analysis revealed enrichment in actin cytoskeleton regulation, focal adhesion, and Ras signaling pathways.
Identification of 9 core regulatory genes (6 mRNAs, 3 circRNAs), highlighting circ_014154 as a key player.
circRNAs identified to potentially function as miRNA sponges regulating osteoblast differentiation.

Conclusions:

This study elucidates the molecular mechanisms of circRNAs in osteoblast differentiation under simulated microgravity.
It offers a novel perspective on the regulatory functions of circRNAs in bone formation.
Findings contribute to understanding bone adaptation to microgravity and potential therapeutic targets.