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Updated: Dec 9, 2025

DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering
Published on: November 9, 2017
Magnetic particle transport through organogel - an application to DNA extraction
Tetsuo Ohashi1, Hiroki Kuyama1
1Technology Research Laboratory, Shimadzu Corporation, 3-9-4 Hikaridai, Seika-cho, Soraku-gun, Kyoto, 619-0237, Japan.
Abstract:
An observation that magnetic particles are transported through organogel encouraged us to investigate its feasibility of liquid-phase displacement in DNA extraction using magnetic particles. Organogel for this study was prepared from a gelator, 12-hydroxystearic acid (12-HSA), and an apolar solvent, methylphenylsilicone oil. The organogel is a gel-like solid material with hydrophobic and elastic properties. These properties, hydrophobicity, and elasticity were demonstrated to be advantageous for liquid compartmentalization and efficient liquid-phase displacement. The extracted DNA with using the organogel device was successfully detected off-chip by conventional real-time PCR.
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