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Updated: Dec 9, 2025

Detection of Foodborne Bacterial Pathogens from Individual Filth Flies
Published on: February 13, 2015
Next-Day Salmonella spp. Detection Method Based on Real-Time PCR for Foods
David Rodríguez-Lázaro1, Marta Hernández2,3
1Microbiology Section, Faculty of Sciences, University of Burgos, Burgos, Spain. drlazaro@ubu.es.
Abstract:
Molecular techniques such as real-time polymerase chain reaction (qPCR) have become a very effective alternative in food microbiology diagnostic for rapid and specific detection of foodborne pathogens such as Salmonella in foods and food-related environments. qPCR is a simple, sensitive, specific, and reproducible assay. Here, we describe the application of real-time PCR-based methods for a rapid (less than 24 h) detection of Salmonella in different types of foods fully compatible with the international standard for detection of Salmonella in food (ISO 6579-1:2017).

