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Related Experiment Video

Updated: Dec 9, 2025

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TRIM24-RIP3 axis perturbation accelerates osteoarthritis pathogenesis.

Jimin Jeon1,2,3, Hyun-Jin Noh2,4, Hyemi Lee1,2,3

  • 1Department of Pharmacology, Ajou University School of Medicine, Suwon, Gyeonggi-do, Republic of Korea.

Annals of the Rheumatic Diseases
|September 8, 2020
PubMed
Summary

Necroptosis, regulated by receptor-interacting protein kinase-3 (RIP3), is implicated in osteoarthritis (OA). Inhibiting the TRIM24-RIP3 axis with AZ-628 shows promise for treating OA by blocking RIP3 kinase activity.

Keywords:
arthritisosteoarthritistherapeutics

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Area of Science:

  • Cellular biology
  • Molecular mechanisms of disease
  • Rheumatology

Background:

  • Necroptosis, a programmed cell death pathway, is increasingly studied in arthritis.
  • The role of receptor-interacting protein kinase-3 (RIP3) in necroptosis and its potential involvement in osteoarthritis (OA) pathogenesis are not fully understood.

Purpose of the Study:

  • To investigate the role of the RIP3 signaling axis in the pathogenesis of osteoarthritis.
  • To identify potential therapeutic targets within the RIP3 pathway for OA treatment.

Main Methods:

  • Experimental osteoarthritis (OA) was induced in wild-type and Rip3 knockout mice.
  • RIP3 expression was analyzed in human OA cartilage.
  • TRIM24, a negative regulator of RIP3, was identified.
  • RIP3 inhibitors were identified using Connectivity Map (CMap) and in silico binding assays.

Main Results:

  • RIP3 expression was significantly elevated in OA cartilage from human patients.
  • RIP3 overexpression exacerbated OA, while Rip3 depletion attenuated OA pathogenesis in mice.
  • TRIM24 knockdown upregulated RIP3, promoting OA, whereas TRIM24 downregulation protected against OA.
  • AZ-628 was identified as a potent RIP3 inhibitor that abrogated OA pathogenesis by inhibiting RIP3 kinase activity.

Conclusions:

  • Perturbation of the TRIM24-RIP3 axis promotes OA chronicity through RIP3 kinase activation.
  • Targeting the TRIM24-RIP3 pathway offers a potential therapeutic strategy for osteoarthritis.