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Thrombospondin is present in articular cartilage and is synthesized by articular chondrocytes
1Department of Musculoskeletal Research, Cleveland Clinic Foundation Research Institute 44195.
Biochemical and Biophysical Research Communications
|June 16, 1988
Abstract:
Thrombospondin, a multifunctional adhesive glycoprotein originally identified in platelets, was isolated and identified from an extract of ovine articular cartilage. Immunoreactive material from a cartilage extract comigrated on gel electrophoresis with purified human platelet thrombospondin. When articular chondrocytes were cultured in the presence of 35S-methionine, metabolically labeled thrombospondin was immunoprecipitated from the culture medium and cell layer extract. These results demonstrate that thrombospondin is present in articular cartilage and is synthesized by articular chondrocytes.