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Transfecting tissue models with CRISPR/Cas9 plasmid DNA using peptide dendrimers
Susanna J Zamolo1, Tamis Darbre, Jean-Louis Reymond
1Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, 3012 Bern, Switzerland. jean-louis.reymond@dcb.unibe.ch.
Chemical Communications (Cambridge, England)
|September 8, 2020
Abstract:
There is currently a lack of efficient reagents to transfect cells with large plasmid DNA, which would be enabling tools for gene editing using CRISPR/Cas9 technology. Herein, we report the discovery of peptide dendrimer Z22 as a non-viral vector for transfecting large CRISPR/Cas9 pDNA into 3D-tumor spheroids with exceptionally high efficiency, low cytotoxicity and low immunogenicity.
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