Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Epigenetic Regulation01:37

Epigenetic Regulation

3.6K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
3.6K
Epigenetic Regulation01:46

Epigenetic Regulation

33.0K
Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
33.0K
Phase II Reactions: Methylation Reactions01:17

Phase II Reactions: Methylation Reactions

565
Methylation is a phase II biotransformation process involving the attachment of a methyl group to a substrate. Enzymes known as methyltransferases orchestrate this reaction.
The mechanism of methylation unfolds in two stages. The first stage sees a methyltransferase enzyme facilitating the transfer of a methyl group from S-adenosylmethionine (SAM) to the substrate, forming S-adenosylhomocysteine (SAH). The second stage involves further metabolism of SAH into homocysteine, which can be recycled...
565
Histone Modification02:32

Histone Modification

15.4K
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
15.4K
Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

9.1K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
9.1K
MicroRNAs01:22

MicroRNAs

3.5K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Post-Transcriptional Regulatory Network of Non-Coding RNAs in Yaks: Molecular Mechanisms of Hypoxia Adaptation and Productive Traits.

Animals : an open access journal from MDPI·2026
Same author

Genome-wide detection and genomic selection based on genotype-by-environment interaction-associated signals for yak coat color.

BMC genomics·2026
Same author

A Comprehensive Analysis of Y-Chromosome Lineages Identifies Paternal Founder Effects in European, African, and Asian Cattle.

Journal of animal breeding and genetics = Zeitschrift fur Tierzuchtung und Zuchtungsbiologie·2026
Same author

Regulation of Myogenic Cell Apoptosis, UPS, and Autophagy During Mammalian Skeletal Myogenesis.

Cells·2026
Same author

Nutritional dynamics and untargeted metabolomics reveal breed and lactation-dependent shifts in goat milk composition: from colostrum to mature milk.

Food chemistry: X·2026
Same author

Single-Cell RNA Sequencing Reveals Dynamic Transcriptional Landscape of Testicular Maturation in Dezhou Donkeys.

Animals : an open access journal from MDPI·2026

Related Experiment Video

Updated: Dec 9, 2025

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
08:56

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Published on: December 5, 2016

11.2K

Insight into m6A methylation from occurrence to functions.

Wenxiu Ru1, Xiaoyan Zhang1, Binglin Yue1

  • 1Key laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, People's Republic of China.

Open Biology
|September 8, 2020
PubMed
Summary

RNA m6A methylation, a key epigenetic mark, regulates gene expression and development. This review covers its discovery, features, and roles in mammals, highlighting ongoing research and unanswered questions.

Keywords:
erasersfunctionsm6A methylationreaderswriters

More Related Videos

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis
08:50

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

Published on: May 14, 2020

7.1K
Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry
08:45

Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry

Published on: April 21, 2022

2.7K

Related Experiment Videos

Last Updated: Dec 9, 2025

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
08:56

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Published on: December 5, 2016

11.2K
Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis
08:50

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

Published on: May 14, 2020

7.1K
Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry
08:45

Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry

Published on: April 21, 2022

2.7K

Area of Science:

  • Molecular Biology
  • Epigenetics
  • Biochemistry

Background:

  • RNA m6A methylation is a crucial post-transcriptional modification.
  • It occurs at the nitrogen-6 position of adenine in various RNA types.
  • This reversible mark is regulated by specific enzymes and binding proteins.

Purpose of the Study:

  • To review the discovery and characteristics of RNA m6A methylation.
  • To introduce mammalian m6A 'writers', 'erasers', and 'readers'.
  • To discuss emerging functions and future research directions.

Main Methods:

  • Literature review of recent studies on RNA m6A.
  • Summary of known enzymes and reader proteins involved in m6A.
  • Discussion of experimental findings and ongoing investigations.

Main Results:

  • m6A modification is widespread across eukaryotic RNA.
  • It plays significant roles in gene expression, development, and cancer.
  • Mammalian m6A machinery involves methyltransferases, demethylases, and reader proteins.

Conclusions:

  • RNA m6A is a vital regulatory mechanism with diverse biological impacts.
  • Further research is needed to fully elucidate its functions and therapeutic potential.
  • Understanding m6A is critical for advancing fields from development to oncology.