Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein and Protein Structure02:15

Protein and Protein Structure

85.5K
Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme...
85.5K
Protein-protein Interfaces02:04

Protein-protein Interfaces

14.3K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
14.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Stiffness Reinforcement in Polymer Networks Through Supramolecular Topological Linking.

Angewandte Chemie (International ed. in English)·2026
Same author

Dilemma-Solving PIC Vesicles: Ligand-Driven Assembly of Stable yet Degradable Nanocarriers for Cytosolic Protein Delivery.

ACS applied materials & interfaces·2026
Same author

Rational Design of Cationic Nanogels with Serum Tolerance for Efficient siRNA Delivery and Antitumor Therapy.

ACS applied materials & interfaces·2025
Same author

Regulated PEGylation of cationic nanogels for enhanced siRNA delivery and orthotopic ovarian tumor therapy.

Journal of controlled release : official journal of the Controlled Release Society·2025
Same author

Tailoring diblock copolymers for efficient siPLK1 delivery and enhanced gene therapy of orthotopic osteosarcoma.

Journal of materials chemistry. B·2025
Same author

Zwitterionic Brush-Grafted Interfacial Bio-Lubricant Evades Complement C3-Mediated Macrophage Phagocytosis for Osteoarthritis Therapy.

Advanced materials (Deerfield Beach, Fla.)·2025

Related Experiment Video

Updated: Dec 9, 2025

Preparation and Friction Force Microscopy Measurements of Immiscible, Opposing Polymer Brushes
13:57

Preparation and Friction Force Microscopy Measurements of Immiscible, Opposing Polymer Brushes

Published on: December 24, 2014

14.2K

Sweet brushes and dirty proteins.

Wouter T E Bosker1, Katja Patzsch2, Martien A Cohen Stuart1

  • 1Laboratory of Physical Chemistry and Colloid Science, Wageningen University, Dreijenplein 6, 6703 HB, Wageningen, The Netherlands.

Soft Matter
|September 9, 2020
PubMed
Summary

Dextran brushes show protein repellency, with high densities creating a homogeneous layer that significantly reduces protein adsorption. However, dextran brushes do not surpass polyethylene oxide brushes in this capability.

More Related Videos

In-vivo Detection of Protein-protein Interactions on Micro-patterned Surfaces
07:42

In-vivo Detection of Protein-protein Interactions on Micro-patterned Surfaces

Published on: March 19, 2010

11.0K
Identification of Post-translational Modifications of Plant Protein Complexes
10:07

Identification of Post-translational Modifications of Plant Protein Complexes

Published on: February 22, 2014

24.4K

Related Experiment Videos

Last Updated: Dec 9, 2025

Preparation and Friction Force Microscopy Measurements of Immiscible, Opposing Polymer Brushes
13:57

Preparation and Friction Force Microscopy Measurements of Immiscible, Opposing Polymer Brushes

Published on: December 24, 2014

14.2K
In-vivo Detection of Protein-protein Interactions on Micro-patterned Surfaces
07:42

In-vivo Detection of Protein-protein Interactions on Micro-patterned Surfaces

Published on: March 19, 2010

11.0K
Identification of Post-translational Modifications of Plant Protein Complexes
10:07

Identification of Post-translational Modifications of Plant Protein Complexes

Published on: February 22, 2014

24.4K

Area of Science:

  • Biomaterials Science
  • Surface Chemistry
  • Polymer Science

Background:

  • Protein adsorption on surfaces is a critical issue in biomaterial applications.
  • Controlling surface properties is key to minimizing unwanted protein interactions.

Purpose of the Study:

  • To investigate the protein repellency of dextran brushes synthesized at various grafting densities.
  • To understand the relationship between dextran brush structure and protein adsorption.

Main Methods:

  • Grafting of dextran onto polystyrene surfaces using polystyrene-dextran block copolymers.
  • Langmuir-Blodgett deposition technique for controlled brush formation.
  • Ellipsometry, water contact-angle measurements, tapping mode AFM, and optical reflectometry for surface characterization and protein adsorption studies.

Main Results:

  • Heterogeneous dextran coatings formed at low grafting densities (<0.20 nm) showed minimal reduction in protein adsorption.
  • Homogeneous dextran brushes formed at higher densities (≥0.20 nm) exhibited significant protein repellency.
  • High grafting densities achieved almost complete protein repellency due to excluded volume interactions.

Conclusions:

  • Dextran brushes can effectively reduce protein adsorption, particularly at high grafting densities, forming a homogeneous layer.
  • The structure of the dextran brush (heterogeneous vs. homogeneous) critically influences its protein-repellent properties.
  • Dextran brushes demonstrate comparable protein adsorption suppression to polyethylene oxide (PEO) brushes.