Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Polybrene technique for red cell antibody screening using microplates.

J A Lown1, J G Ivey

  • 1Department of Haematology, Royal Perth Hospital, Western Australia.

Journal of Clinical Pathology
|May 1, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Platelet monolayer formation in the solid-phase red cell adherence method using fresh frozen plasma.

Immunohematology·2004
Same author

Determination of activated protein C resistance in anticoagulated and lupus positive patients.

Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis·2000
Same author

HPA-1, 3, 5 genotyping to establish a typed platelet donor panel.

Pathology·2000
Same author

Monocyte activation in platelet concentrates.

Vox sanguinis·1998
Same author

White cells in fresh-frozen plasma: evaluation of a new white cell-reduction filter.

Transfusion·1998
Same author

Immobilization of platelets with a cell fixation buffer.

Vox sanguinis·1998

A new microplate antibody screening method using the polybrene technique offers equivalent sensitivity and specificity to conventional tube testing. This adaptation is a useful advancement for pretransfusion diagnostics.

Area of Science:

  • Transfusion Medicine
  • Immunology
  • Clinical Laboratory Science

Background:

  • Accurate antibody screening is crucial for safe pretransfusion testing.
  • Conventional tube testing is a standard method but can be labor-intensive.

Purpose of the Study:

  • To adapt the manual polybrene technique into a microplate format for antibody screening.
  • To evaluate the sensitivity and specificity of the adapted microplate polybrene technique.
  • To compare the microplate technique with conventional tube testing.

Main Methods:

  • The manual polybrene technique was modified for use in a microplate assay.
  • Antibody screening was performed using the adapted microplate technique and conventional tube testing.
  • Various antibodies and serologically inert sera were utilized for comparison.

Related Experiment Videos

  • Sensitivity and specificity were assessed for both methods.

    • Main Results:

    • The microplate polybrene technique demonstrated equivalent results to conventional tube testing.
    • The sensitivity and specificity of the adapted method were comparable to the established technique.
    • No significant differences were observed between the two testing methodologies.

    Conclusions:

    • The adapted polybrene microplate technique is a viable and useful method for antibody screening in pretransfusion testing.
    • This microplate adaptation offers a potentially more efficient alternative to conventional tube testing.
    • The findings support the integration of this technique into routine pretransfusion diagnostics.