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Spleen proteomics data from high fat diet fed mice.

Sara L Svahn1, Bagmi Pattanaik1, Louise Grahnemo1,2

  • 1Dept. of Physiology, Institute of Neuroscience and Physiology, Gothenburg, Sweden.

Data in Brief
|September 9, 2020
PubMed
Summary

Dietary fat composition impacts immune response. High-fat diets rich in polyunsaturated fatty acids improve survival from S. aureus infection compared to saturated fatty acids, affecting spleen protein expression.

Keywords:
DietHigh fat dietLow fat dietPolyunsaturated fatty acidsProteomicsSaturated fatty acidsiTRAQ

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Area of Science:

  • Immunology
  • Proteomics
  • Nutritional Science

Background:

  • Dietary fat composition influences host physiology, including immune function.
  • Previous studies indicated differential survival rates in S. aureus infection based on dietary fat saturation.
  • This study investigates the proteomic changes in spleen tissue in response to different high-fat diets.

Purpose of the Study:

  • To analyze the impact of diet on spleen protein expression.
  • To compare proteomic profiles between mice fed high-fat diets rich in saturated versus polyunsaturated fatty acids.
  • To identify specific proteins affected by dietary fat composition.

Main Methods:

  • Quantitative proteomics using isobaric tags for relative and absolute quantification (iTRAQ) on mouse spleen tissue.
  • Mass spectrometry analysis with Proteome Discoverer software.
  • Statistical analysis of identified proteins using Qlucore Omics Explorer.

Main Results:

  • 924 proteins were identified across all dietary groups (low-fat diet, high-fat saturated, high-fat polyunsaturated).
  • Only 20 proteins showed differential expression when comparing the two high-fat diets (HFD-S vs. HFD-P).
  • No significant protein expression differences were observed between low-fat and high-fat diets.

Conclusions:

  • Dietary fat saturation significantly alters spleen protein expression, particularly between saturated and polyunsaturated fatty acid-rich diets.
  • Specific proteins with inverse expression patterns were identified, suggesting distinct molecular pathways affected by diet.
  • These findings contribute to understanding the molecular mechanisms linking diet and immune response.