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High Ethanol Concentration (77 g/L) of Industrial Hemp Biomass Achieved Through Optimizing the Relationship between
Jikai Zhao1, Youjie Xu1, Weiqun Wang2
1Department of Biological and Agricultural Engineering, Kansas State University, Manhattan, Kansas 66506, United States.
ACS Omega
|September 9, 2020
Summary
Optimizing solid loading in simultaneous saccharification and fermentation (SSF) is key for bioethanol production. Particle size reduction significantly boosted ethanol concentration, improving process efficiency and reducing resource use.
Area of Science:
- Biotechnology and Bioengineering
- Renewable Energy
- Biomass Conversion
Background:
- Simultaneous saccharification and fermentation (SSF) is a crucial process for converting biomass into bioethanol.
- Optimizing solid loading is essential for maximizing ethanol yield and concentration while minimizing costs.
- Hemp biomass, an abundant lignocellulosic material, is a promising feedstock for bioethanol production.
Purpose of the Study:
- To investigate the relationship between solid loading and bioethanol yield/concentration in SSF of alkali-pretreated hemp biomass.
- To determine the optimal solid loading strategy to enhance ethanol titer and avoid random selection.
- To evaluate the impact of enzyme dosage, Tween 80 addition, and particle size reduction on SSF performance.
Main Methods:
- Alkali-pretreated hemp biomass was subjected to SSF under four different conditions.
- Enzyme dosages (cellulase and hemicellulase) and Tween 80 addition were varied.
- Particle size reduction of the biomass was implemented in one scenario.
- Statistical models (linear and quadratic) were used to correlate solid loading with ethanol yield and concentration.
Main Results:
- Bioethanol yield showed a negative linear correlation (R² = 0.76-0.93) with solid loading (6-21%).
- Bioethanol concentration exhibited a negative quadratic correlation (R² = 0.96-0.99) with solid loading.
- Particle size reduction (Case IV) resulted in the highest ethanol concentration (77 g/L) by extending the "solid effect".
Conclusions:
- Understanding the relationship between solid loading and ethanol production is vital for efficient SSF.
- Particle size reduction is a promising strategy to enhance ethanol concentration and overcome limitations associated with high solid loading.
- Optimized solid loading strategies can reduce enzyme and water consumption, leading to more cost-effective bioethanol production.
