Overexpression of miR-150-5p Alleviates Apoptosis in Sepsis-Induced Myocardial Depression
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Updated: Dec 9, 2025
Murine Myocardial Infarction Model using Permanent Ligation of Left Anterior Descending Coronary Artery
Published on: August 16, 2019
Xiao Geng Zhu1, Tie Ning Zhang1, Ri Wen1
1Department of Pediatrics, Shengjing Hospital of China Medical University, Shenyang, Liaoning Province 110004, China.
Abstract:
Sepsis-induced myocardial depression has high mortality and is very common in intensive care units. Previous studies have found that microRNAs play an important role in regulating sepsis-induced myocardial depression. miR-150-5p is involved in many biological processes; however, the mechanism underlying its role in sepsis-induced myocardial depression is still unclear. In this study, we generated rat models of septic shock induced by lipopolysaccharide. Whole genomic RNA sequencing was performed on 12 left ventricles collected after LPS treatment to identify miRNAs. Most of the target genes of the differently expressed microRNAs were involved in apoptosis, according to Gene Ontology. We also observed apoptosis in the heart tissue and in H9C2 cardiomyocytes stimulated with lipopolysaccharide, indicating that cell apoptosis may be an important mechanism in sepsis-induced myocardial depression. Furthermore, the expression of miR-150-5p was reduced, and overexpression of miR-150-5p with mimics resulted in a decrease in apoptosis, decreased expression of cleaved caspase3 and Bax, and increased expression of Bcl-2. Additionally, after H9C2 cells were transfected with miR-150-5p mimics or an inhibitor, the expression of Akt2 decreased or increased, respectively. These findings suggest that miR-150-5p can alleviate apoptosis and may be a novel therapeutic target for sepsis-induced myocardial depression.
Insights
MicroRNA-150-5p (miR-150-5p) alleviates sepsis-induced myocardial depression by reducing heart cell apoptosis. This finding suggests miR-150-5p as a potential therapeutic target for critical care patients.
Area of Science:
- Cardiovascular Biology
- Molecular Medicine
- Critical Care Medicine
Background:
- Sepsis-induced myocardial depression is a life-threatening condition common in intensive care units.
- MicroRNAs are implicated in regulating this condition, but the specific role of miR-150-5p remains unclear.
Purpose of the Study:
- To investigate the mechanism of miR-150-5p in sepsis-induced myocardial depression.
- To explore miR-150-5p as a potential therapeutic target.
Main Methods:
- Lipopolysaccharide (LPS) induced septic shock in rat models.
- Whole genomic RNA sequencing identified differentially expressed microRNAs.
- Apoptosis was assessed in heart tissue and H9C2 cardiomyocytes.
- miR-150-5p mimics and inhibitors were used to evaluate its functional role.
Main Results:
- LPS treatment induced apoptosis in cardiac cells.
- miR-150-5p expression was significantly reduced in septic hearts.
- Overexpression of miR-150-5p decreased apoptosis, cleaved caspase-3, and Bax, while increasing Bcl-2.
- miR-150-5p regulated Akt2 expression in cardiomyocytes.
Conclusions:
- miR-150-5p plays a protective role by alleviating sepsis-induced myocardial apoptosis.
- miR-150-5p represents a promising novel therapeutic target for sepsis-induced myocardial depression.
