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Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins
Published on: August 31, 2019
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Nanoliposomal encapsulation of saffron bioactive compounds; characterization and optimization.
Roxana Hadavi1, Seid Mahdi Jafari1, Iman Katouzian1
1Department of Food Materials and Process Design Engineering, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
International Journal of Biological Macromolecules
|September 10, 2020
Summary
This study optimized saffron-loaded nanoliposomes for enhanced encapsulation and stability. The ideal formulation included 0.15% saffron, 35% sunflower oil, and 1% lecithin for superior size and color.
Area of Science:
- Pharmaceutical Nanotechnology
- Drug Delivery Systems
- Lipid Nanocarriers
Background:
- Nanoliposomes are crucial lipid-based nanocarriers for delivering hydrophilic and hydrophobic bioactive compounds.
- Saffron extract possesses valuable bioactive properties but requires effective delivery systems.
- Optimizing nanoliposome formulations is key to maximizing the potential of bioactive ingredients.
Purpose of the Study:
- To formulate and characterize saffron-loaded nanoliposomes.
- To investigate the impact of lecithin, saffron extract, and sunflower oil concentrations on nanoliposome properties.
- To determine the optimal formulation for enhanced encapsulation efficiency, stability, size, and color.
Main Methods:
- Formulation of nanoliposomes using varying concentrations of lecithin, saffron extract, and sunflower oil.
- Evaluation of encapsulation efficiency, creaming stability, particle size, and color indices.
- Application of second-order polynomial models and multiple linear regression analysis for optimization.
- Characterization using Dynamic Light Scattering (DLS) and Transmission Electron Microscopy (TEM).
Main Results:
- High R-squared values (0.950-0.989) indicated model accuracy for predicting nanoliposome properties.
- Increased oil and saffron content negatively impacted creaming stability due to insufficient lecithin.
- Optimized nanoliposomes exhibited sizes around 100 nm with a narrow size distribution (PDI).
- TEM confirmed spherical, homogenous nanoliposomes with smooth surfaces.
- Lecithin and saffron concentration significantly affected color indices, while sunflower oil did not.
Conclusions:
- The optimal formulation for saffron-loaded nanoliposomes was determined to be 0.15% saffron, 35% sunflower oil, and 1% lecithin.
- This optimized formulation demonstrated superior encapsulation efficiency, particle size, and color characteristics.
- The study provides a robust methodology for optimizing nanoliposome formulations for bioactive delivery.
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