Related Experiment Video

Updated: Dec 9, 2025

Time-Lapse Video Microscopy for Assessment of EYFP-Parkin Aggregation as a Marker for Cellular Mitophagy
09:29

Time-Lapse Video Microscopy for Assessment of EYFP-Parkin Aggregation as a Marker for Cellular Mitophagy

Published on: May 4, 2016

7.5K

FOXO3a-dependent Parkin regulates the development of gastric cancer by targeting ATP-binding cassette transporter E1

Dan Ding1,2, Xiang Ao1,2, Mengyang Li1

  • 1School of Basic Medical Sciences, College of Medicine, Qingdao University, Qingdao, China.

Journal of Cellular Physiology
|September 11, 2020
PubMed

Insights

This study reveals a new pathway involving FOXO3a, Parkin, and ABCE1 in gastric cancer (GC). This FOXO3a-Parkin-ABCE1 axis impacts GC cell growth, movement, and cell death, offering a potential new therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Gastric cancer (GC) is a leading cause of cancer death in China.
  • Parkin functions as a tumor suppressor, but its mechanism in GC is not fully understood.
  • Forkhead box O3a (FOXO3a) is a key regulator of cancer cell behavior.

Purpose of the Study:

  • To elucidate the mechanism of Parkin in gastric cancer.
  • To identify the regulatory pathway involving FOXO3a, Parkin, and ABCE1 in GC.
  • To explore potential therapeutic targets for GC.

Main Methods:

  • Observing Parkin expression in GC cells and patient tissues.
  • Investigating the effects of doxorubicin (DOX) on Parkin expression and GC cells.
  • Analyzing the regulatory relationship between FOXO3a, Parkin, and ABCE1 at the transcriptional level.

Main Results:

  • Parkin expression was low in GC cells and tissues; Parkin inhibited GC cell proliferation and migration.
  • Doxorubicin (DOX) increased Parkin expression and enhanced its anti-cancer effects.
  • FOXO3a suppressed GC cell proliferation and migration while promoting apoptosis by regulating Parkin transcription.
  • Parkin inhibited proliferation and migration and promoted apoptosis by downregulating ABCE1 expression.

Conclusions:

  • A novel regulatory axis, FOXO3a-Parkin-ABCE1, was identified in gastric cancer.
  • This axis significantly modulates GC cell proliferation, migration, and apoptosis.
  • The FOXO3a-Parkin-ABCE1 pathway represents a potential therapeutic target for gastric cancer treatment.
Keywords:
ABCE1FOXO3aParkindoxorubicingastric cancer

More Related Videos

Protection of H9c2 Myocardial Cells from Oxidative Stress by Crocetin via PINK1/Parkin Pathway-Mediated Mitophagy07:40

Protection of H9c2 Myocardial Cells from Oxidative Stress by Crocetin via PINK1/Parkin Pathway-Mediated Mitophagy

Published on: May 26, 2023

1.5K
Author Spotlight: Genetic Profiling for Fluorouracil Response in Gastric Cancer06:21

Author Spotlight: Genetic Profiling for Fluorouracil Response in Gastric Cancer

Published on: May 10, 2024

1.0K

Related Concept Videos

mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.5K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
5.0K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.0K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
15.7K