Expression level and diagnostic value of exosomal NEAT1/miR-204/MMP-9 in acute ST-segment elevation myocardial...

Area of Science:

• Biochemistry
• Molecular Biology
• Cardiology

Background:

• Acute myocardial infarction (AMI) is a leading cause of cardiovascular mortality.
• Myocardial repair after infarction is crucial to prevent heart failure.
• Exosomes, as intercellular messengers, have shown potential in repairing infarcted myocardium.

Purpose of the Study:

• To investigate the association between serum exosomal long non-coding RNA NEAT1 and AMI.
• To explore the underlying mechanisms involving NEAT1, miR-204, and MMP-9 in AMI.
• To evaluate the diagnostic potential of these molecules for acute ST-segment elevation myocardial infarction (STEMI).

Main Methods:

• Serum exosomes were isolated from control, unstable angina (UA), and STEMI groups.
• Quantitative real-time PCR (qRT-PCR) was used to detect lncRNA NEAT1 and miR-204 expression.
• Western blot analysis was performed to measure MMP-9 levels.
• Spearman correlation, logistic regression, and ROC curve analysis were employed.

Main Results:

• lncRNA NEAT1 and MMP-9 expressions were significantly upregulated in serum exosomes of STEMI patients and positively correlated.
• miR-204 expression was downregulated in STEMI patients.
• No significant correlation was found between miR-204 and NEAT1 or MMP-9.
• Exosomal NEAT1, miR-204, and MMP-9 demonstrated diagnostic value for STEMI.

Conclusions:

• Serum exosomal NEAT1 and MMP-9 are upregulated and correlated in STEMI.
• Downregulation of miR-204 is observed in STEMI patients.
• Exosomal NEAT1, miR-204, and MMP-9 serve as promising biomarkers for the diagnosis of acute ST-segment elevation myocardial infarction.