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Menthol based hydrophobic deep eutectic solvent for extraction and purification of ergosterol using response surface
Lalit Khare1, Tanvi Karve1, Ratnesh Jain2
1Department of Pharmaceutical Sciences & Technology, Institute of Chemical Technology, N.P. Marg, Matunga, Mumbai 400019, Maharashtra, India.
Food Chemistry
|September 13, 2020
Summary
Researchers developed a green extraction method for ergosterol from mushrooms using deep eutectic solvents. This ergosterol was converted to vitamin D2, offering a potential solution for vitamin D deficiency.
Area of Science:
- Biochemistry
- Green Chemistry
- Food Science
Background:
- Vitamin D deficiency is a global health concern linked to numerous diseases.
- Fortified foods and supplements are common strategies to address vitamin D deficiency.
Purpose of the Study:
- To explore green extraction of ergosterol from mushrooms using hydrophobic deep eutectic solvents.
- To optimize extraction parameters and convert ergosterol to vitamin D2 (ergocalciferol).
Main Methods:
- Screened 39 hydrophobic deep eutectic solvents for ergosterol extraction.
- Optimized extraction parameters (time, solvent volume) using response surface methodology (RSM).
- Purified ergosterol and converted it to vitamin D2 via UV radiation.
Main Results:
- Achieved a maximum ergosterol yield of 6995.00 µg/g dry weight mushroom using menthol: pyruvic acid solvent.
- The extraction solvent demonstrated reusability for six cycles with up to 28% retained efficiency.
- Obtained a final yield of 2142.01 µg/g dry weight mushroom for vitamin D2 (ergocalciferol).
Conclusions:
- Menthol: pyruvic acid deep eutectic solvent is effective for green ergosterol extraction from mushrooms.
- The developed method provides a sustainable route for vitamin D2 production.
- This approach offers a viable alternative for addressing vitamin D deficiency through natural sources.
