Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

19.6K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
19.6K
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

11.6K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
11.6K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.0K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.0K
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

8.5K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
8.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multiplexed optoacoustic tracking and magnetic actuation of labeled blood cells in living mice.

Science advances·2026
Same author

Transcranial pulse stimulation modulates spectral signatures of Alzheimer's disease in the 3×Tg-AD mouse model.

Alzheimer's research & therapy·2026
Same author

Data-driven super-resolution optoacoustic imaging via physically encoded signal acquisition.

Research square·2026
Same author

Quantitative in-vivo full-waveform ultrasound tomography workflow integrating reflection imaging and resolution analysis.

Physics in medicine and biology·2026
Same author

Localization-based techniques for super-resolution imaging of vascular dynamics.

Innovation (Cambridge (Mass.))·2026
Same author

Divergent scalp-to-region distance alteration patterns in autism spectrum disorders, Parkinson's disease and Alzheimer's disease.

bioRxiv : the preprint server for biology·2026
Same journal

Two-photon 3D imaging of optically stimulated neural activity at 100 Hz.

Light, science & applications·2026
Same journal

Quasi-bound states in the continuum driven photoresponse in multiple quantum wells for machine vision.

Light, science & applications·2026
Same journal

Spin-photon qubits for scalable quantum network.

Light, science & applications·2026
Same journal

Dual-mode switchable and reconfigurable Van der Waals phototransistor for multi-state image encryption.

Light, science & applications·2026
Same journal

Weak polarization electric field Ⅲ-N LEDs on polar plane with enhanced efficiency and strong lateral carrier confinement.

Light, science & applications·2026
Same journal

Bi-layer photonic random meta-composite for cryogenic thermal control by ultra-broadband scattering matched reflectance.

Light, science & applications·2026
See all related articles

Related Experiment Video

Updated: Dec 9, 2025

Three-dimensional Optical-resolution Photoacoustic Microscopy
08:31

Three-dimensional Optical-resolution Photoacoustic Microscopy

Published on: May 3, 2011

18.7K

Multifocal structured illumination optoacoustic microscopy.

Zhenyue Chen1, Ali Özbek1, Johannes Rebling1

  • 1Institute for Biomedical Engineering and Institute of Pharmacology and Toxicology, University of Zurich and ETH Zurich, Zurich, Switzerland.

Light, Science & Applications
|September 14, 2020
PubMed
Summary
This summary is machine-generated.

Multifocal structured illumination optoacoustic microscopy (MSIOAM) enables real-time 3D imaging by scanning a grid of laser spots. This breakthrough achieves fast, high-resolution imaging for dynamic biological processes.

Keywords:
Imaging and sensingMicroscopy

More Related Videos

Switchable Acoustic and Optical Resolution Photoacoustic Microscopy for In Vivo Small-animal Blood Vasculature Imaging
10:17

Switchable Acoustic and Optical Resolution Photoacoustic Microscopy for In Vivo Small-animal Blood Vasculature Imaging

Published on: June 26, 2017

12.3K
Multispectral Optoacoustic Tomography for Functional Imaging in Vascular Research
06:40

Multispectral Optoacoustic Tomography for Functional Imaging in Vascular Research

Published on: June 8, 2022

2.2K

Related Experiment Videos

Last Updated: Dec 9, 2025

Three-dimensional Optical-resolution Photoacoustic Microscopy
08:31

Three-dimensional Optical-resolution Photoacoustic Microscopy

Published on: May 3, 2011

18.7K
Switchable Acoustic and Optical Resolution Photoacoustic Microscopy for In Vivo Small-animal Blood Vasculature Imaging
10:17

Switchable Acoustic and Optical Resolution Photoacoustic Microscopy for In Vivo Small-animal Blood Vasculature Imaging

Published on: June 26, 2017

12.3K
Multispectral Optoacoustic Tomography for Functional Imaging in Vascular Research
06:40

Multispectral Optoacoustic Tomography for Functional Imaging in Vascular Research

Published on: June 8, 2022

2.2K

Area of Science:

  • Biomedical Imaging
  • Optics and Photonics
  • Microscopy

Background:

  • Optoacoustic (OA) imaging bridges macro and micro scales.
  • Current high-resolution OA microscopy is limited by slow point-by-point scanning, restricting speed and field of view.

Purpose of the Study:

  • Introduce multifocal structured illumination optoacoustic microscopy (MSIOAM) for real-time 3D imaging.
  • Overcome the speed and field-of-view limitations of conventional OA microscopy.

Main Methods:

  • Shaped the excitation laser beam into a grid of focused spots using a diffraction grating and condenser.
  • Scanned the multifocal spots using an acousto-optic deflector at kHz rates for rapid imaging.

Main Results:

  • Achieved real-time 3D imaging with a 15 Hz frame rate.
  • Demonstrated a 10 mm wide volumetric field of view with 28 μm spatial resolution in phantom and in vivo mouse studies.

Conclusions:

  • MSIOAM significantly enhances imaging speed and field of view in optoacoustic microscopy.
  • The method is poised to advance biological investigations of dynamic processes across multiple scales.