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Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging
Published on: January 5, 2015
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A Facile and Reproducible Synthesis of Near-Infrared Fluorescent Conjugates with Small Targeting Molecules for
Friederike Reeßing1,2, Mafalda Bispo3, Marina López-Álvarez3
1Department of Radiology, Medical Imaging Center, University of Groningen, University Medical Center Groningen, Hanzeplein 1, Groningen 9713GZ, The Netherlands.
ACS Omega
|September 14, 2020
Summary
Researchers developed new fluorescent tracers for optical imaging of microbial infections. These targeted probes enable early diagnosis and treatment of bacterial and fungal infections, aiding in the fight against antimicrobial resistance.
Area of Science:
- Biomedical imaging
- Organic chemistry
- Microbiology
Background:
- Optical imaging offers high sensitivity for detecting microbial infections.
- Near-infrared (NIR) probes allow deeper tissue penetration for imaging.
- Antimicrobial resistance necessitates novel diagnostic and therapeutic strategies.
Purpose of the Study:
- To develop a general strategy for conjugating IRdye fluorescent dyes to small molecules for targeted infection imaging.
- To create novel fluorescent probes for bacterial and fungal infections.
- To explore applications in optical imaging and photodynamic therapy.
Main Methods:
- Conjugation of IRdye800CW and IRdye700DX to vancomycin and amphotericin B using coupling agents (HBTU/HATU).
- Utilizing precipitation for purification of the synthesized conjugates.
- In vitro testing of synthesized compounds, such as Vanco-800CW, for selectivity.
Main Results:
- Achieved high yields (over 50%) in conjugation reactions.
- Demonstrated effective purification using precipitation.
- Confirmed high in vitro selectivity of the Vanco-800CW model compound.
Conclusions:
- A versatile platform for creating targeted fluorescent tracers for infection imaging was established.
- These novel agents offer potential for improved diagnostics and theranostics.
- The developed tracers are valuable tools for combating antimicrobial resistance through early infection detection.
