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LINC00893 inhibits papillary thyroid cancer by suppressing AKT pathway via stabilizing PTEN

Shujing Li1, Yanyan Zhang1, Jian Dong1

  • 1Department of General Surgery, First Hospital of Shanxi Medical University, Taiyuan, Shanxi, China.

Cancer Biomarkers : Section a of Disease Markers
|September 14, 2020
PubMed

Insights

Long non-coding RNA LINC00893 acts as a tumor suppressor in papillary thyroid carcinoma (PTC). Its overexpression inhibits cancer cell proliferation and migration by upregulating PTEN and blocking the AKT pathway.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) play critical roles in human cancer development.
  • LINC00893 has been identified as lowly expressed in thyroid carcinoma (THCA) via GEPIA.
  • The specific function of LINC00893 in papillary thyroid carcinoma (PTC) remains uncharacterized.

Purpose of the Study:

  • To investigate the role and mechanism of LINC00893 in papillary thyroid carcinoma (PTC).
  • To determine the effect of LINC00893 on PTC cell proliferation and migration.
  • To elucidate the molecular pathway modulated by LINC00893 in PTC.

Main Methods:

  • GEPIA analysis to assess LINC00893 expression in THCA.
  • Gain-of-function assays to evaluate the impact of LINC00893 overexpression on PTC cells.
  • Signal transduction reporter array to identify modulated pathways.
  • Western blotting and mRNA stability assays to confirm molecular mechanisms.
  • Rescue assays to validate the functional role of LINC00893 in PTC.

Main Results:

  • LINC00893 was confirmed to be lowly expressed in PTC cells.
  • Overexpression of LINC00893 significantly inhibited PTC cell proliferation and migration.
  • LINC00893 was found to modulate the PTEN/AKT pathway.
  • LINC00893 overexpression led to increased PTEN expression and decreased phosphorylated AKT levels.
  • LINC00893 stabilizes PTEN mRNA by recruiting FUS protein.
  • LINC00893 suppressed PTC progression via the PTEN/AKT pathway.

Conclusions:

  • LINC00893 functions as a tumor suppressor in papillary thyroid carcinoma (PTC).
  • LINC00893 inhibits PTC proliferation and migration by upregulating PTEN and consequently inhibiting the AKT pathway.
  • This study provides novel insights into the regulatory role of LINC00893 in thyroid cancer.
Keywords:
FUSLINC00893PTEN/AKT pathwaypapillary thyroid cancer

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