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Cell surface-associated structural proteins in connective tissue cells.

P Bornstein, J F Ash

    Proceedings of the National Academy of Sciences of the United States of America
    |June 1, 1977
    PubMed
    Summary
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    Fibroblasts display a cell surface meshwork of collagen and fibronectin. This external protein network is crucial for cell attachment and interaction with the extracellular matrix.

    Area of Science:

    • Cell Biology
    • Biochemistry
    • Extracellular Matrix Research

    Background:

    • Fibroblasts play a critical role in tissue structure and repair.
    • Cell surface proteins like collagen and fibronectin are vital for cell adhesion and communication.
    • Understanding the organization of these proteins is key to deciphering cellular functions.

    Purpose of the Study:

    • To investigate the presence and arrangement of collagen and fibronectin on the surface of normal rat kidney fibroblasts.
    • To determine the mobility of these cell surface proteins in attached versus suspended cells.
    • To propose a model for the function of these proteins in cell-substrate and extracellular matrix interactions.

    Main Methods:

    • Indirect immunofluorescence using affinity-purified antibodies to collagen.

    Related Experiment Videos

  • Observation of cell surface protein distribution and mobility in attached and suspended fibroblast cultures.
  • Morphological analysis of protein patterns.

    • Main Results:

    • Collagen and/or procollagen were detected on the surface of kidney fibroblasts in a reticular pattern.
    • Cell surface collagen exhibited restricted translational movement in attached cells.
    • Fibronectin (LETS protein) showed similar surface localization and restricted mobility.
    • These proteins were lost upon cell dissociation and regained upon reattachment.

    Conclusions:

    • Fibroblasts possess an external meshwork composed of collagen and fibronectin on their cell surface.
    • This cell surface protein network is likely involved in mediating cell attachment to substrata.
    • The meshwork may play a significant role in cellular interactions with the extracellular matrix.