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Life After Death
Kavita Gaur1, Puja Sakhuja1, Kaushik Majumdar1
1Department of Pathology, GB Pant Institute of Postgraduate Medical Education and Research (GIPMER), New Delhi, India.
American Journal of Clinical Pathology
|September 15, 2020
Summary
Many expired primary immunohistochemistry antibodies retain functionality, with nearly half showing optimal staining. Pathologists should assess antibody performance rather than relying solely on expiration dates for quality control.
Area of Science:
- Immunohistochemistry
- Histopathology
- Diagnostic Pathology
Background:
- Primary antibodies are crucial reagents in immunohistochemistry (IHC).
- Expiration dates are set by manufacturers, but antibody stability may extend beyond these dates.
- Assessing the performance of expired antibodies is essential for laboratory efficiency and cost-effectiveness.
Purpose of the Study:
- To evaluate the performance characteristics of expired primary immunohistochemistry antibodies.
- To compare the functionality of expired antibodies with fresh ones when possible.
Main Methods:
- Sixty-nine expired primary antibodies were tested for specificity, background staining, and intensity.
- Optimal staining was defined as 2+ or 3+ intensity with intact specificity and minimal background.
- Suboptimal results included deviations from normal staining patterns, such as splotchy or granular staining.
Main Results:
- Nearly half (49.2%) of the expired antibodies demonstrated optimal staining results.
- A small percentage (10.1%) of expired antibodies showed equivalent performance to fresh antibodies of the same clone.
- 11.6% of expired antibodies exhibited suboptimal staining patterns like splotchy or granular results.
Conclusions:
- A significant proportion of primary immunohistochemistry antibodies maintain functionality after their expiration date.
- Pathologists' assessment of antibody performance is more critical than manufacturer-specified expiration dates.
- Balancing histopathologic performance with economic considerations is key for quality maintenance in IHC.
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