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HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
Published on: March 31, 2019
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Nucleotide sequencing of the HoxA gene cluster using Gorilla fosmid clones
Takashi Kitano1, Choong-Gon Kim1, Naruya Saitou1
1Division of Population Genetics, National Institute of Genetics, Japan.
Journal of Genomics
|September 16, 2020
Abstract:
We sequenced the western gorilla (Gorilla gorilla) HoxA cluster region using seven fosmid clones, and found that the total tiling path sequence was 214,185 bp from the 5' non-genic region of HoxA1 to the 3' non-genic region of Evx1. We compared the nucleotide sequence with the gorilla genome sequence in the NCBI database, and the overall proportion of nucleotide difference was estimated to be 0.0005-0.0007. These estimates are lower than overall genomic polymorphism in gorillas.

