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Insight on adsorption of cellulase on wet ground corncob residues and its evaluation by multivariate linear analysis
Wei Liu1, Qian Ren2, Ruijie Wu2
1Tianjin Key Laboratory of Pulp & Paper, Tianjin University of Science & Technology, Tianjin 300457, China; State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, China; Department of Chemical Engineering, University of New Brunswick, Fredericton, New Brunswick E3B 5A3, Canada.
Abstract:
Understanding the adsorption behavior and the interaction between substrates and enzymes are critical to improving enzymatic hydrolysis efficiency and reducing bioconversion cost. Herein, the adsorption of cellulase on wet ground corncob residues was studied, and the effects of main characteristics of wet ground corncob residues on adsorption capacity were quantitatively analyzed with the combination of principal component analysis and multiple linear regression models. The results showed that the adsorption of cellulase on wet ground corncob residues was fitted well with Langmuir isotherm adsorption and pseudo second-order kinetics model, the adsorption rate and adsorption capacity were greatly enhanced with increasing grinding time; the multiple linear regression models describing the relationship between main characteristics of corncob residues and adsorption capacity to cellulase were established; the significance of these characteristics were in the following order: average particle size, crystallinity index, specific surface area, surface lignin concentration, water retention value, and surface charge density.
