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Updated: Dec 8, 2025

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
Published on: September 2, 2021
Adding to the CASeload: unwarranted p53 signaling induced by Cas9
Veronica Rendo1, Oana M Enache2, Uri Ben-David3
1Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA, USA.
Abstract:
We investigated the genetic and transcriptional changes associated with Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) associated protein 9 (Cas9) expression in human cancer cell lines. For a subset of cell lines with a wild-type tumor protein TP53 (best known as p53), we detected p53 pathway activation, DNA damage accumulation and emerging p53-inactivating mutations following Cas9 introduction. We discuss the potential implications of our findings in basic and translational research.
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