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Air-Liquid Interface In Vitro Models for Respiratory Toxicology Research: Consensus Workshop and Recommendations
Ghislaine Lacroix1, Wolfgang Koch2, Detlef Ritter2
1Chronic Risks Division, Institut National de l'Environnement Industriel et des RISques, Verneuil-en-Halatte, France.
Applied in Vitro Toxicology
|September 21, 2020
Summary
Air-liquid interface (ALI) models offer a promising in vitro approach for inhalation toxicology, bridging the gap between traditional cell cultures and animal testing. Further validation is needed for regulatory use.
Area of Science:
- In vitro toxicology
- Respiratory research
- Cell culture models
Background:
- Air-liquid interface (ALI) models are intermediate in relevance between submerged in vitro and in vivo models for inhalation toxicology.
- ALI methods can complement or replace traditional in vitro and in vivo approaches in specific contexts.
- Limited validation studies hinder data comparison and regulatory acceptance of current ALI methods.
Purpose of the Study:
- To assess the current status of in vitro air-liquid interface (ALI) models for respiratory toxicology.
- To discuss potential validation strategies for ALI inhalation models.
- To foster collaboration among academic, commercial, and regulatory stakeholders.
Main Methods:
- A 1-day workshop in Paris (March 2016) focused on ALI in vitro models for respiratory toxicology.
- The workshop included presentations and an expert panel discussion on validation approaches.
- Over 60 representatives from European academic, commercial, and regulatory organizations attended.
Main Results:
- A significant need for validation studies for ALI inhalation models was identified.
- Current ALI methods are not yet suitable for regulatory assessments.
- Discussions highlighted the challenges in comparing data across different ALI methodologies.
Conclusions:
- Recommendations were made regarding approaches to validation studies for ALI inhalation models.
- The workshop outcomes underscore the need for standardized validation to enable regulatory use.
- Further research and collaborative efforts are crucial for advancing ALI models in inhalation toxicology.
