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Updated: Dec 8, 2025
An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function
Published on: May 4, 2021
Integrative Analysis of MicroRNAs and mRNAs in LPS-Induced Macrophage Inflammation Based on Adipose Tissue Stem Cell
Xiaozhi Bai1, Ting He1, Mingchuan Liu2
1Department of Burns and Cutaneous Surgery, Xijing Hospital, Fourth Military Medical University, No. 127 West Changle Road, Xi'an, 710032, Shaanxi, China.
Abstract:
Severe inflammation can lead to multiple organ dysfunction syndrome, which has high mortality. Adipose-derived stem cells have been shown to affect the inflammatory response of macrophages. However, the molecular mechanism of the anti-inflammatory capacity of adipose-derived stem cells (ADSCs) remains to be understood. In the present study, a macrophage inflammation model was established by LPS, and treated with different volumes of ADSC supernatant. Then, we investigated the key genes in the LPS group and treatment group by RT-PCR, RNA sequencing technology, and bioinformatics analysis. A total of 26 miRNAs and 11,882 mRNAs were differentially expressed between them. The expression of 15 of the miRNAs (9 upregulated and 6 downregulated) was confirmed by RT-PCR. GO and KEGG pathway analyses of the targets of the 9 significantly upregulated miRNAs showed that they were related to immune system process, inflammatory response, lipopolysaccharide, and TNF-α, NF-κB, Toll-like receptor, and MAPK signaling pathways. Moreover, a miRNA-mRNA network also revealed 8 important genes (Mapkapk2, Sepp1, Cers6, Snn, ZfP568, Ccdc93, Pofut1, Pik3cd). We finally confirmed the expression of these 8 targeted genes by performing the RT-PCR analysis. This study may provide a new understanding of the molecular mechanism of ADSCs in the inflammatory response related to multiple miRNAs and mRNAs.
Insights
Adipose-derived stem cells (ADSCs) can reduce inflammation by altering macrophage responses. This study identified key microRNAs (miRNAs) and messenger RNAs (mRNAs) involved in ADSC-mediated anti-inflammatory effects, revealing potential molecular mechanisms.
Area of Science:
- Biomedical research
- Stem cell biology
- Immunology
Background:
- Severe inflammation can cause multiple organ dysfunction syndrome (MODS) with high mortality.
- Adipose-derived stem cells (ADSCs) modulate macrophage inflammatory responses, but their molecular mechanisms are unclear.
Purpose of the Study:
- To elucidate the molecular mechanisms underlying the anti-inflammatory effects of ADSCs on macrophages.
- To identify key microRNAs (miRNAs) and messenger RNAs (mRNAs) involved in ADSC-mediated inflammation modulation.
Main Methods:
- Established a lipopolysaccharide (LPS)-induced macrophage inflammation model.
- Treated macrophages with ADSC supernatant.
- Utilized RT-PCR, RNA sequencing, and bioinformatics analysis to identify differentially expressed genes.
- Constructed a miRNA-mRNA network.
Main Results:
- Identified 26 differentially expressed miRNAs and 11,882 differentially expressed mRNAs.
- Confirmed expression of 15 miRNAs (9 upregulated, 6 downregulated) via RT-PCR.
- GO and KEGG pathway analyses linked upregulated miRNAs to immune responses and inflammatory signaling pathways (e.g., TNF-α, NF-κB, MAPK).
- A miRNA-mRNA network highlighted 8 key genes (Mapkapk2, Sepp1, Cers6, Snn, ZfP568, Ccdc93, Pofut1, Pik3cd).
Conclusions:
- ADSCs exert anti-inflammatory effects through complex interactions involving multiple miRNAs and mRNAs.
- This study provides novel insights into the molecular basis of ADSC-mediated immunomodulation.
- Identified potential therapeutic targets for managing inflammatory conditions.
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