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Zerumbone Restores Gut Microbiota Composition in ETBF Colonized AOM/DSS Mice
Hye-Won Cho1, Ki-Jong Rhee2, Yong-Bin Eom1,3
1Department of Medical Sciences, College of Medical Sciences, Soonchunhyang University, Asan 31538, Republic of Korea.
Journal of Microbiology and Biotechnology
|September 22, 2020
Summary
Zerumbone treatment restored intestinal microbiota balance in mice with colorectal cancer (CRC) models. This compound influenced microbial diversity and richness, suggesting potential for gut health restoration.
Area of Science:
- Microbiology
- Gastroenterology
- Pharmacology
Background:
- Colorectal cancer (CRC) is a major global health concern.
- Gut microbiota dysbiosis, particularly involving enterotoxigenic Bacteroides fragilis (ETBF), is linked to CRC development.
- Restoring gut microbial balance is a potential therapeutic strategy for CRC.
Purpose of the Study:
- To investigate the effect of zerumbone on intestinal microbiota composition in a mouse model of CRC.
- To determine if zerumbone can restore gut microbial balance disrupted by ETBF colonization and chemical induction.
Main Methods:
- Utilized a mouse model colonized with ETBF and induced with azoxymethane (AOM)/dextran sulphate sodium (DSS).
- Administered zerumbone (60 mg/kg) to experimental groups.
- Analyzed gut microbiota composition using 16S rRNA gene sequencing (V3-V4 region).
- Employed Linear Discriminant Analysis of Effect Size (LEfSe) for microbial analysis.
Main Results:
- Zerumbone treatment significantly altered microbiota composition compared to untreated ETBF-colonized mice.
- Increased abundance of Bacteroides, including B. fragilis, was observed in zerumbone-treated groups.
- Microbial diversity and richness, initially decreased in the CRC model, were enhanced by zerumbone.
Conclusions:
- Zerumbone demonstrates potential in restoring the balance of intestinal microbiota.
- The findings suggest zerumbone's therapeutic utility in modulating gut microbial composition for CRC management.
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