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Viral Infectivity Quantification and Neutralization Assays Using Laser Force Cytology
Colin G Hebert1, Keya L Rodrigues2, Nicole DiNardo3
1LumaCyte, LLC, Charlottesville, VA, USA. colin.hebert@lumacyte.com.
Methods in Molecular Biology (Clifton, N.J.)
|September 22, 2020
Summary
Laser Force Cytology (LFC) offers a rapid, label-free method to measure viral infectivity in cells. This technology provides a faster, more robust alternative to traditional assays for vaccine development and viral safety testing.
Area of Science:
- Biotechnology
- Cell Biology
- Virology
Background:
- Traditional viral infectivity assays like TCID50 and plaque assays are time-consuming and user-dependent.
- Accurate viral infectivity assessment is crucial for vaccine development, production, and viral safety testing.
Purpose of the Study:
- To introduce Laser Force Cytology (LFC) as a novel, rapid, and quantitative method for assessing viral infectivity.
- To demonstrate the application of LFC for various infectivity measurements, including titer determination, in-process monitoring, and neutralization assays.
Main Methods:
- LumaCyte's Radiance instrument utilizes Laser Force Cytology (LFC), combining optics and microfluidics to analyze single cells.
- LFC measures intrinsic biophysical and biochemical properties (e.g., refractive index, stiffness) without labels.
- Data from LFC is used to quantify viral infectivity in cell populations.
Main Results:
- LFC provides rapid and robust infectivity measurements.
- The technology enables label-free analysis of cellular changes due to viral infection.
- Experimental designs are presented for LFC applications replacing traditional methods.
Conclusions:
- LFC offers a significant advancement over traditional viral infectivity assays.
- This technology facilitates faster and more reliable assessments in vaccine development and viral safety.
- LFC has broad applications in bioprocessing and diagnostics requiring infectivity measurements.
