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Updated: Dec 8, 2025

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale
Published on: August 16, 2018
Structure elucidation of a new triterpene from Inonotus obliquus
Sangmin Park1, Hyeji Shin1, Dawon Park1
1College of Pharmacy, Korea University, Sejong, Republic of Korea.
Abstract:
Ethyl acetate (EtOAc) fraction of the Inonotus obliquus (Hymenochaetaceae) significantly inhibited nitric oxide production in lipopolysaccharide (LPS)-induced murine BV2 microglial cells. A new triterpene, characterized as inonotusol H (1), was isolated from the EtOAc fraction using the bioactivity-guided fractionation together with four known triterpenes, inotodiol (2), trametenolic acid (3), inonotsutriols A (4), and inonotusol A (5). Among them, Compounds 2-4 significantly reduced LPS-induced nitric oxide production to 4.5 ± 0.8%, 47.4 ± 4.4%, and 2.8 ± 1.7%, respectively, at a concentration of 30 μM.
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