Click chemistry as a tool in biosensing systems for sensitive copper detection

Copper detection for diagnostic purposes is an appealing field due to the important biological role copper plays as a trace metal. A convenient strategy for sensing copper is to utilize its catalytic ability. Therefore, this review summarizes approaches for copper determination by Cu^I-catalyzed azide/alkyne cycloaddition (CuAAC). The concept was introduced in 2006 and all contributions made up to the middle of 2020 are covered in this review. The issue is divided into three categories: electrochemical, visual, and fluorescence-based methods. The advantages, as well as the disadvantages, of every group, are discussed in detail. The methodology which allows for the determination of copper content in water and human biological samples from 5 s up to 48 h without complex instrumentation are discussed. The reported range of limit of detection (LOD) was 0.38 aM-20 μM, with 1-10 nM being the typical range. The most successful strategies involved using DNA chains or enzymes in the sensing systems.